Gene description for Slit3
Gene name slit homolog 3 (Drosophila)
Gene symbol Slit3
Other names/aliases Slil2
Slit1
b2b2362.1Clo
Species Mus musculus
 Database cross references - Slit3
ExoCarta ExoCarta_20564
Vesiclepedia VP_20564
Entrez Gene 20564
UniProt Q9WVB4  
 Slit3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slit3
Molecular Function
    signaling receptor binding GO:0005102 TAS
    calcium ion binding GO:0005509 IEA
    heparin binding GO:0008201 IBA
    Roundabout binding GO:0048495 IBA
    Roundabout binding GO:0048495 IEA
    Roundabout binding GO:0048495 ISO
Biological Process
    aortic valve morphogenesis GO:0003180 IMP
    atrioventricular valve morphogenesis GO:0003181 IMP
    axon guidance GO:0007411 IBA
    axon guidance GO:0007411 IDA
    axon guidance GO:0007411 ISO
    negative regulation of cell population proliferation GO:0008285 IGI
    animal organ morphogenesis GO:0009887 IGI
    animal organ morphogenesis GO:0009887 IMP
    negative regulation of gene expression GO:0010629 IGI
    negative regulation of cell growth GO:0030308 IEA
    negative regulation of cell growth GO:0030308 ISO
    cellular response to hormone stimulus GO:0032870 IEA
    Roundabout signaling pathway GO:0035385 IEA
    Roundabout signaling pathway GO:0035385 ISO
    axon extension involved in axon guidance GO:0048846 IEA
    axon extension involved in axon guidance GO:0048846 ISO
    negative chemotaxis GO:0050919 IBA
    negative chemotaxis GO:0050919 IEA
    negative chemotaxis GO:0050919 ISO
    response to cortisol GO:0051414 IEA
    ventricular septum morphogenesis GO:0060412 IMP
    apoptotic process involved in luteolysis GO:0061364 IEA
    negative regulation of chemokine-mediated signaling pathway GO:0070100 IEA
    negative regulation of chemokine-mediated signaling pathway GO:0070100 ISO
Subcellular Localization
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified Slit3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slit3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Thbs2 21826
Co-fractionation Mus musculus
2 Ighmbp2 20589
Co-localization Mus musculus
3 Robo4  
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
4 Robo1  
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Slit3 is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
Developmental Biology IEA Reactome
Nervous system development IEA Reactome
Signaling by ROBO receptors IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here