Gene description for Slit3
Gene name slit homolog 3 (Drosophila)
Gene symbol Slit3
Other names/aliases Slil2
Slit1
b2b2362.1Clo
Species Mus musculus
 Database cross references - Slit3
ExoCarta ExoCarta_20564
Vesiclepedia VP_20564
Entrez Gene 20564
UniProt Q9WVB4  
 Slit3 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slit3
Molecular Function
    Roundabout binding GO:0048495 ISO
    receptor binding GO:0005102 TAS
    calcium ion binding GO:0005509 IEA
Biological Process
    negative regulation of cell growth GO:0030308 ISO
    negative regulation of chemokine-mediated signaling pathway GO:0070100 ISO
    axon extension involved in axon guidance GO:0048846 ISO
    multicellular organismal development GO:0007275 IEA
    Roundabout signaling pathway GO:0035385 ISO
    negative chemotaxis GO:0050919 ISO
    axon guidance GO:0007411 ISO
    organ morphogenesis GO:0009887 IMP
    nervous system development GO:0007399 IEA
    negative regulation of cell proliferation GO:0008285 IGI
    cell differentiation GO:0030154 IEA
    negative regulation of gene expression GO:0010629 IGI
Subcellular Localization
    extracellular space GO:0005615 ISO
    mitochondrion GO:0005739 ISO
    membrane GO:0016020 ISO
    extracellular region GO:0005576 IEA
 Experiment description of studies that identified Slit3 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slit3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Slit3 is involved
PathwayEvidenceSource
Netrin-1 signaling IEA Reactome





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