Gene description for Thbs2
Gene name thrombospondin 2
Gene symbol Thbs2
Other names/aliases TSP2
Thbs-2
Species Mus musculus
 Database cross references - Thbs2
ExoCarta ExoCarta_21826
Vesiclepedia VP_21826
Entrez Gene 21826
UniProt Q03350  
 Thbs2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Thbs2
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IEA
Biological Process
    cell adhesion GO:0007155 IEA
    negative regulation of angiogenesis GO:0016525 IBA
    negative regulation of angiogenesis GO:0016525 IMP
    negative regulation of angiogenesis GO:0016525 ISO
    positive regulation of synapse assembly GO:0051965 IEA
    positive regulation of synapse assembly GO:0051965 ISO
Subcellular Localization
    extracellular region GO:0005576 IEA
    basement membrane GO:0005604 IDA
    extracellular matrix GO:0031012 IDA
    platelet alpha granule GO:0031091 IEA
    platelet alpha granule GO:0031091 ISO
    collagen-containing extracellular matrix GO:0062023 IBA
 Experiment description of studies that identified Thbs2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Thbs2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Thbs2 21826
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 Thbs1 21825
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 Slit3 20564
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here