Gene description for Thbs1
Gene name thrombospondin 1
Gene symbol Thbs1
Other names/aliases TSP-1
TSP1
Thbs-1
tbsp1
Species Mus musculus
 Database cross references - Thbs1
ExoCarta ExoCarta_21825
Vesiclepedia VP_21825
Entrez Gene 21825
 Thbs1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Thbs1
Molecular Function
    integrin binding GO:0005178 ISO
    protein binding GO:0005515 IPI
    transforming growth factor beta binding GO:0050431 ISO
    fibroblast growth factor binding GO:0017134 ISO
    fibronectin binding GO:0001968 ISO
    fibrinogen binding GO:0070051 ISO
    calcium ion binding GO:0005509 IEA
    collagen V binding GO:0070052 ISO
    extracellular matrix binding GO:0050840 IDA
    phosphatidylserine binding GO:0001786 ISO
    laminin binding GO:0043236 ISO
    heparin binding GO:0008201 ISO
    low-density lipoprotein particle binding GO:0030169 ISO
Biological Process
    negative regulation of endothelial cell proliferation GO:0001937 ISO
    blood vessel morphogenesis GO:0048514 IGI
    negative regulation of dendritic cell antigen processing and presentation GO:0002605 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of plasminogen activation GO:0010757 ISO
    positive regulation of macrophage chemotaxis GO:0010759 IMP
    endocardial cushion development GO:0003197 IGI
    negative regulation of endothelial cell migration GO:0010596 ISO
    positive regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902043 ISO
    negative regulation of fibrinolysis GO:0051918 ISO
    negative regulation of nitric oxide mediated signal transduction GO:0010751 ISO
    peptide cross-linking GO:0018149 ISO
    response to glucose GO:0009749 ISO
    response to magnesium ion GO:0032026 ISO
    positive regulation of phosphorylation GO:0042327 ISO
    cellular response to nitric oxide GO:0071732 IMP
    positive regulation of blood vessel endothelial cell migration GO:0043536 ISO
    positive regulation of macrophage activation GO:0043032 ISO
    positive regulation of transforming growth factor beta production GO:0071636 ISO
    growth plate cartilage development GO:0003417 IMP
    behavioral response to pain GO:0048266 IMP
    positive regulation of endothelial cell migration GO:0010595 ISO
    activation of MAPK activity GO:0000187 ISO
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISO
    positive regulation of reactive oxygen species metabolic process GO:2000379 ISO
    negative regulation of cell-matrix adhesion GO:0001953 ISO
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 ISO
    positive regulation of translation GO:0045727 ISO
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 ISO
    negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II GO:0002581 ISO
    negative regulation of blood vessel endothelial cell migration GO:0043537 ISO
    negative regulation of endothelial cell chemotaxis GO:2001027 ISO
    positive regulation of angiogenesis GO:0045766 ISO
    outflow tract morphogenesis GO:0003151 IGI
    engulfment of apoptotic cell GO:0043652 ISO
    negative regulation of plasma membrane long-chain fatty acid transport GO:0010748 ISO
    positive regulation of tumor necrosis factor biosynthetic process GO:0042535 ISO
    cell migration GO:0016477 ISO
    response to mechanical stimulus GO:0009612 ISO
    positive regulation of blood coagulation GO:0030194 ISO
    response to calcium ion GO:0051592 ISO
    positive regulation of cell-substrate adhesion GO:0010811 IDA
    inflammatory response GO:0006954 ISO
    negative regulation of angiogenesis GO:0016525 ISO
    response to endoplasmic reticulum stress GO:0034976 IMP
    positive regulation of cell migration GO:0030335 ISO
    negative regulation of interleukin-12 production GO:0032695 ISO
    cell adhesion GO:0007155 IEA
    positive regulation of endothelial cell apoptotic process GO:2000353 ISO
    negative regulation of cGMP-mediated signaling GO:0010754 ISO
    response to unfolded protein GO:0006986 IEA
    sprouting angiogenesis GO:0002040 ISO
    positive regulation of protein kinase B signaling GO:0051897 ISO
    positive regulation of chemotaxis GO:0050921 ISO
    positive regulation of fibroblast migration GO:0010763 ISO
    regulation of cGMP metabolic process GO:0030823 ISO
    cell cycle arrest GO:0007050 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    fibrinogen complex GO:0005577 ISO
    extracellular matrix GO:0031012 ISO
    endoplasmic reticulum GO:0005783 IDA
    extracellular space GO:0005615 ISO
    secretory granule GO:0030141 ISO
    extracellular region GO:0005576 IEA
    external side of plasma membrane GO:0009897 ISO
    platelet alpha granule GO:0031091 ISO
    cell surface GO:0009986 ISO
    cytoplasm GO:0005737 ISO
    sarcoplasmic reticulum GO:0016529 IDA
 Experiment description of studies that identified Thbs1 in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 39
MISEV standards
IEM
EV Biophysical techniques
TSG101|HSC70|FLOT1
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Thbs1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Thbs1 is involved
No pathways found





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