Gene description for Tubb4a
Gene name tubulin, beta 4A class IVA
Gene symbol Tubb4a
Other names/aliases AI325297
M(beta)4
Tubb
Tubb4
Species Mus musculus
 Database cross references - Tubb4a
ExoCarta ExoCarta_22153
Entrez Gene 22153
UniProt Q9D6F9  
 Tubb4a identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
 Gene ontology annotations for Tubb4a
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IEA
    GTP binding GO:0005525 IEA
    nucleotide binding GO:0000166 IEA
    GTPase activity GO:0003924 IEA
Biological Process
    microtubule-based process GO:0007017 IEA
    protein polymerization GO:0051258 IEA
    cell projection organization GO:0030030 IEA
Subcellular Localization
    internode region of axon GO:0033269 IDA
    cell projection GO:0042995 IDA
    cilium GO:0005929 IDA
    neuronal cell body GO:0043025 IDA
    myelin sheath GO:0043209 IDA
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IDA
    protein complex GO:0043234 IEA
    cytoskeleton GO:0005856 IEA
    microtubule GO:0005874 ISO
 Experiment description of studies that identified Tubb4a in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Tubb4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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