Gene description for Tubb4a
Gene name tubulin, beta 4A class IVA
Gene symbol Tubb4a
Other names/aliases AI325297
M(beta)4
Tubb
Tubb4
Species Mus musculus
 Database cross references - Tubb4a
ExoCarta ExoCarta_22153
Vesiclepedia VP_22153
Entrez Gene 22153
UniProt Q9D6F9  
 Tubb4a identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
 Gene ontology annotations for Tubb4a
Molecular Function
    GTPase activity GO:0003924 IEA
    structural constituent of cytoskeleton GO:0005200 IBA
    structural constituent of cytoskeleton GO:0005200 IEA
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    mitotic cell cycle GO:0000278 IBA
    cytoskeleton organization GO:0007010 IEA
    microtubule-based process GO:0007017 IEA
    cell projection organization GO:0030030 IEA
    negative regulation of microtubule polymerization GO:0031115 IEA
    negative regulation of microtubule polymerization GO:0031115 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    cilium GO:0005929 IDA
    axoneme GO:0005930 IEA
    axoneme GO:0005930 ISO
    microtubule cytoskeleton GO:0015630 ISO
    internode region of axon GO:0033269 IDA
    cell projection GO:0042995 IDA
    neuronal cell body GO:0043025 IDA
    myelin sheath GO:0043209 HDA
    myelin sheath GO:0043209 IDA
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
 Experiment description of studies that identified Tubb4a in exosomes
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Tubb4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bmpr2 12168
Co-fractionation Mus musculus
2 Tjp1  
Co-fractionation Mus musculus
3 Ubc  
Reconstituted Complex Mus musculus
4 SNCA 6622
Affinity Capture-MS Homo sapiens
5 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
6 Tuba4a 22145
Co-fractionation Mus musculus
7 Sstr3  
Proximity Label-MS Mus musculus
8 Thrb  
Affinity Capture-MS Mus musculus
9 HACE1 57531
Affinity Capture-MS Homo sapiens
10 Tubgcp2  
Co-fractionation Mus musculus
11 MTNR1A  
Reconstituted Complex Homo sapiens
12 PRKACB  
Affinity Capture-MS Bos taurus
13 MAPT  
Reconstituted Complex Homo sapiens
14 Unk  
Affinity Capture-RNA Mus musculus
15 Camsap3  
Co-fractionation Mus musculus
16 Lrrk2  
Affinity Capture-Western Mus musculus
17 Fancd2  
Affinity Capture-MS Mus musculus
18 Kctd13  
Affinity Capture-MS Mus musculus
19 MTNR1B  
Reconstituted Complex Homo sapiens
20 Lipc  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Tubb4a is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Aggrephagy IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Autophagy IEA Reactome
Axon guidance IEA Reactome
Carboxyterminal post-translational modifications of tubulin IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
COPI-dependent Golgi-to-ER retrograde traffic IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
COPI-mediated anterograde transport IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G2/M Transition IEA Reactome
Gap junction assembly IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Interferon Signaling IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Intraflagellar transport IEA Reactome
Kinesins IEA Reactome
L1CAM interactions IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Macroautophagy IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
MHC class II antigen presentation IEA Reactome
Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Nervous system development IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
PKR-mediated signaling IEA Reactome
Post-translational protein modification IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Recycling pathway of L1 IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
Sealing of the nuclear envelope (NE) by ESCRT-III IEA Reactome
Selective autophagy IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome
Transport of connexons to the plasma membrane IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





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