Gene description for Usp9x
Gene name ubiquitin specific peptidase 9, X chromosome
Gene symbol Usp9x
Other names/aliases 5730589N07Rik
AA407302
AA407699
AL022658
AL022749
Dffrx
FAF-X
Fafl
Species Mus musculus
 Database cross references - Usp9x
ExoCarta ExoCarta_22284
Entrez Gene 22284
UniProt P70398  
 Usp9x identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Usp9x
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 IBA
    hydrolase activity GO:0016787 IEA
    cysteine-type peptidase activity GO:0008234 IEA
    protein binding GO:0005515 IPI
    co-SMAD binding GO:0070410 ISO
    ubiquitinyl hydrolase activity GO:0036459 IEA
    peptidase activity GO:0008233 IEA
    ubiquitin-specific protease activity GO:0004843 IBA
Biological Process
    cell cycle GO:0007049 IEA
    BMP signaling pathway GO:0030509 ISO
    regulation of proteasomal protein catabolic process GO:0061136 IBA
    axon extension GO:0048675 ISO
    cellular response to transforming growth factor beta stimulus GO:0071560 IMP
    post-embryonic development GO:0009791 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    protein deubiquitination GO:0016579 ISO
    proteolysis GO:0006508 IEA
    in utero embryonic development GO:0001701 IGI
    neuron migration GO:0001764 ISO
    hippocampus development GO:0021766 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    neuron projection extension GO:1990138 IGI
    mitotic nuclear division GO:0007067 IEA
    cell division GO:0051301 IEA
    cerebellar cortex structural organization GO:0021698 IMP
    chromosome segregation GO:0007059 IEA
Subcellular Localization
    growth cone GO:0030426 ISO
    cell projection GO:0042995 IEA
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
    apical part of cell GO:0045177 IDA
 Experiment description of studies that identified Usp9x in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Usp9x
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Usp9x is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here