Gene description for Atp1a3
Gene name ATPase, Na+/K+ transporting, alpha 3 polypeptide
Gene symbol Atp1a3
Other names/aliases Atpa-2
Species Mus musculus
 Database cross references - Atp1a3
ExoCarta ExoCarta_232975
Entrez Gene 232975
UniProt Q6PIC6  
 Atp1a3 identified in exosomes derived from the following tissue/cell type
Microglia 16081791    
Oligodendrocytes 21136642    
 Gene ontology annotations for Atp1a3
Molecular Function
    sodium:potassium-exchanging ATPase activity GO:0005391 ISO
    sodium:potassium-exchanging ATPase activity involved in regulation of cardiac muscle cell membrane potential GO:0086037 IGI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
    heparan sulfate proteoglycan binding GO:0043395 IPI
    hydrolase activity GO:0016787 IEA
    D1 dopamine receptor binding GO:0031748 ISO
    nucleotide binding GO:0000166 IEA
    chaperone binding GO:0051087 ISO
Biological Process
    sodium ion transport GO:0006814 ISO
    transport GO:0006810 IEA
    cellular sodium ion homeostasis GO:0006883 ISO
    ionotropic glutamate receptor signaling pathway GO:0035235 IMP
    potassium ion transmembrane transport GO:0071805 IGI
    adult locomotory behavior GO:0008344 IMP
    cellular potassium ion homeostasis GO:0030007 ISO
    cardiac muscle contraction GO:0060048 IDA
    potassium ion transport GO:0006813 ISO
    memory GO:0007613 IMP
    regulation of cardiac muscle cell membrane potential GO:0086036 IGI
    sodium ion export from cell GO:0036376 ISO
    response to drug GO:0042493 IMP
    ion transport GO:0006811 IEA
    visual learning GO:0008542 IMP
    sodium ion transmembrane transport GO:0035725 IGI
    potassium ion import GO:0010107 ISO
Subcellular Localization
    dendritic spine head GO:0044327 ISO
    sarcolemma GO:0042383 IDA
    myelin sheath GO:0043209 ISO
    synapse GO:0045202 ISO
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 ISO
    axon GO:0030424 ISO
    Golgi apparatus GO:0005794 ISO
    sodium:potassium-exchanging ATPase complex GO:0005890 ISO
    nucleus GO:0005634 IDA
    extracellular vesicle GO:1903561 ISO
    membrane GO:0016020 IEA
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 IDA
    dendritic spine neck GO:0044326 ISO
 Experiment description of studies that identified Atp1a3 in exosomes
1
Experiment ID 14
ISEV standards
EM
EV Biophysical techniques
RAB7|RAB11
EV Cytosolic markers
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
DNM
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Atp1a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atp1a3 is involved
PathwayEvidenceSource
Ion transport by P-type ATPases IEA Reactome





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