Gene description for ACSL6
Gene name acyl-CoA synthetase long-chain family member 6
Gene symbol ACSL6
Other names/aliases ACS2
FACL6
LACS 6
LACS2
LACS5
Species Homo sapiens
 Database cross references - ACSL6
ExoCarta ExoCarta_23305
Vesiclepedia VP_23305
Entrez Gene 23305
HGNC 16496
MIM 604443
UniProt Q9UKU0  
 ACSL6 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for ACSL6
Molecular Function
    long-chain fatty acid-CoA ligase activity GO:0004467 IDA
    long-chain fatty acid-CoA ligase activity GO:0004467 IMP
    long-chain fatty acid-CoA ligase activity GO:0004467 NAS
    long-chain fatty acid-CoA ligase activity GO:0004467 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    enzyme binding GO:0019899 IPI
    protein homodimerization activity GO:0042803 IDA
    arachidonate-CoA ligase activity GO:0047676 IBA
    arachidonate-CoA ligase activity GO:0047676 ISS
Biological Process
    very long-chain fatty acid metabolic process GO:0000038 IBA
    long-chain fatty acid metabolic process GO:0001676 IBA
    long-chain fatty acid metabolic process GO:0001676 IDA
    long-chain fatty acid metabolic process GO:0001676 IMP
    acyl-CoA metabolic process GO:0006637 NAS
    neuroblast proliferation GO:0007405 IEA
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 IBA
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 TAS
Subcellular Localization
    mitochondrial outer membrane GO:0005741 IEA
    peroxisomal membrane GO:0005778 IEA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 NAS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
 Experiment description of studies that identified ACSL6 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACSL6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACSL6 23305
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
2 ZNF655  
Two-hybrid Homo sapiens
3 CUL4A 8451
Affinity Capture-MS Homo sapiens
4 PRPH2 5961
Affinity Capture-MS Homo sapiens
5 PCMTD2  
Two-hybrid Homo sapiens
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