Gene description for Ppia
Gene name peptidylprolyl isomerase A
Gene symbol Ppia
Other names/aliases 2700098C05
Cphn
CyP-18
CypA
Species Mus musculus
 Database cross references - Ppia
ExoCarta ExoCarta_268373
Vesiclepedia VP_268373
Entrez Gene 268373
UniProt P17742  
 Ppia identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppia
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IMP
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 ISO
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 ISS
    integrin binding GO:0005178 IDA
    protein binding GO:0005515 IPI
    cyclosporin A binding GO:0016018 IBA
    cyclosporin A binding GO:0016018 ISO
    RNA polymerase II CTD heptapeptide repeat P3 isomerase activity GO:0140839 IEA
    RNA polymerase II CTD heptapeptide repeat P6 isomerase activity GO:0140840 IEA
    heparan sulfate binding GO:1904399 ISO
    heparan sulfate binding GO:1904399 ISS
Biological Process
    protein peptidyl-prolyl isomerization GO:0000413 ISO
    protein peptidyl-prolyl isomerization GO:0000413 ISS
    negative regulation of protein phosphorylation GO:0001933 ISO
    negative regulation of protein phosphorylation GO:0001933 ISS
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of protein phosphorylation GO:0001934 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein folding GO:0006457 IBA
    protein folding GO:0006457 IEA
    negative regulation of protein kinase activity GO:0006469 ISO
    negative regulation of protein kinase activity GO:0006469 ISS
    apoptotic process GO:0006915 ISO
    apoptotic process GO:0006915 ISS
    platelet activation GO:0030168 IMP
    neuron differentiation GO:0030182 IMP
    neutrophil chemotaxis GO:0030593 ISO
    neutrophil chemotaxis GO:0030593 ISS
    leukocyte chemotaxis GO:0030595 ISO
    leukocyte chemotaxis GO:0030595 ISS
    activation of protein kinase B activity GO:0032148 ISO
    activation of protein kinase B activity GO:0032148 ISS
    negative regulation of stress-activated MAPK cascade GO:0032873 ISO
    negative regulation of stress-activated MAPK cascade GO:0032873 ISS
    lipid droplet organization GO:0034389 IEA
    lipid droplet organization GO:0034389 ISO
    cellular response to oxidative stress GO:0034599 ISO
    cellular response to oxidative stress GO:0034599 ISS
    endothelial cell activation GO:0042118 ISO
    endothelial cell activation GO:0042118 ISS
    positive regulation of MAPK cascade GO:0043410 ISO
    positive regulation of MAPK cascade GO:0043410 ISS
    regulation of viral genome replication GO:0045069 ISO
    regulation of viral genome replication GO:0045069 ISS
    positive regulation of viral genome replication GO:0045070 IEA
    positive regulation of viral genome replication GO:0045070 ISO
    positive regulation of protein secretion GO:0050714 IEA
    positive regulation of protein secretion GO:0050714 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    cell adhesion molecule production GO:0060352 ISO
    cell adhesion molecule production GO:0060352 ISS
    negative regulation of protein K48-linked ubiquitination GO:0061944 ISO
    negative regulation of protein K48-linked ubiquitination GO:0061944 ISS
    platelet aggregation GO:0070527 IMP
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISO
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISS
    negative regulation of viral life cycle GO:1903901 IEA
    negative regulation of viral life cycle GO:1903901 ISO
    regulation of apoptotic signaling pathway GO:2001233 ISO
    regulation of apoptotic signaling pathway GO:2001233 ISS
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular region GO:0005576 ISS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISS
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Ppia in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppia
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBM7  
Two-hybrid Homo sapiens
2 Mbd2  
Reconstituted Complex Mus musculus
3 Rps23 66475
Co-fractionation Mus musculus
4 Hdac1 433759
Affinity Capture-Western Mus musculus
5 Rplp0 11837
Co-fractionation Mus musculus
6 Tmem173  
Proximity Label-MS Mus musculus
7 Ddx58  
Affinity Capture-Western Mus musculus
8 Rps28 54127
Co-fractionation Mus musculus
9 Eed  
Affinity Capture-MS Mus musculus
10 SPATS2L 26010
Two-hybrid Homo sapiens
11 MAPT  
Reconstituted Complex Homo sapiens
12 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
13 Tfe3  
Affinity Capture-MS Mus musculus
14 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
15 Atg16l1  
Affinity Capture-MS Mus musculus
16 Rplp2 67186
Co-fractionation Mus musculus
17 Rpl18a 76808
Co-fractionation Mus musculus
18 Mavs  
Affinity Capture-Western Mus musculus
19 Homer1  
Affinity Capture-MS Mus musculus
20 Kcnma1  
Affinity Capture-MS Mus musculus
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