Gene description for Map4k4
Gene name mitogen-activated protein kinase kinase kinase kinase 4
Gene symbol Map4k4
Other names/aliases 9430080K19Rik
AU043147
AU045934
AW046177
HGK
Nik
Species Mus musculus
 Database cross references - Map4k4
ExoCarta ExoCarta_26921
Entrez Gene 26921
UniProt P97820  
 Map4k4 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Map4k4
Molecular Function
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    kinase activity GO:0016301 IEA
    transferase activity GO:0016740 IEA
    protein serine/threonine kinase activity GO:0004674 ISO
    creatine kinase activity GO:0004111 ISO
    nucleotide binding GO:0000166 IEA
    protein kinase activity GO:0004672 IEA
    ATP binding GO:0005524 ISO
Biological Process
    regulation of JNK cascade GO:0046328 ISO
    phosphorylation GO:0016310 IEA
    regulation of apoptotic process GO:0042981 IBA
    negative regulation of insulin secretion involved in cellular response to glucose stimulus GO:0061179 ISO
    negative regulation of neuron projection regeneration GO:0070571 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    protein phosphorylation GO:0006468 ISO
    intracellular signal transduction GO:0035556 ISO
    regulation of mitotic cell cycle GO:0007346 IBA
    neuron projection morphogenesis GO:0048812 IBA
    activation of protein kinase activity GO:0032147 IBA
    MAPK cascade GO:0000165 IBA
    stress-activated protein kinase signaling cascade GO:0031098 IBA
    negative regulation of neuron projection development GO:0010977 ISO
    response to stress GO:0006950 IDA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    intracellular GO:0005622 IDA
 Experiment description of studies that identified Map4k4 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Map4k4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Map4k4 is involved
No pathways found





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