Gene description for Tubb5
Gene name tubulin, beta 5 class I
Gene symbol Tubb5
Other names/aliases Tubb
Species Rattus norvegicus
 Database cross references - Tubb5
ExoCarta ExoCarta_29214
Entrez Gene 29214
UniProt P69897  
 Tubb5 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Cortical neurones 16446100    
Hepatocytes 19367702    
 Gene ontology annotations for Tubb5
Molecular Function
    protein complex binding GO:0032403 IDA
    MHC class I protein binding GO:0042288 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    GTP binding GO:0005525 IEA
    GTPase activity GO:0003924 IEA
    protein domain specific binding GO:0019904 IPI
    protein binding GO:0005515 IPI
    GTPase activating protein binding GO:0032794 IPI
    structural constituent of cytoskeleton GO:0005200 ISO
Biological Process
    protein polymerization GO:0051258 IEA
    microtubule-based process GO:0007017 ISO
    biological_process GO:0008150 ND
    metabolic process GO:0008152 IEA
    spindle assembly GO:0051225 ISO
    cellular process GO:0009987 ISO
Subcellular Localization
    protein complex GO:0043234 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    microtubule GO:0005874 ISO
    nuclear envelope lumen GO:0005641 ISO
    cell body GO:0044297 ISO
 Experiment description of studies that identified Tubb5 in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 30
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
FLOT
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Tubb5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here