Gene description for Tubb5
Gene name tubulin, beta 5 class I
Gene symbol Tubb5
Other names/aliases Tubb
Species Rattus norvegicus
 Database cross references - Tubb5
ExoCarta ExoCarta_29214
Vesiclepedia VP_29214
Entrez Gene 29214
UniProt P69897  
 Tubb5 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Cortical neurones 16446100    
Hepatocytes 19367702    
 Gene ontology annotations for Tubb5
Molecular Function
    GTPase activity GO:0003924 IEA
    structural constituent of cytoskeleton GO:0005200 IBA
    structural constituent of cytoskeleton GO:0005200 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    protein domain specific binding GO:0019904 IPI
    ubiquitin protein ligase binding GO:0031625 ISO
    GTPase activating protein binding GO:0032794 IPI
    MHC class I protein binding GO:0042288 ISO
    protein-containing complex binding GO:0044877 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    mitotic cell cycle GO:0000278 IBA
    cytoskeleton organization GO:0007010 IEA
    microtubule-based process GO:0007017 IEA
    microtubule-based process GO:0007017 ISO
    biological_process GO:0008150 ND
    regulation of synapse organization GO:0050807 ISO
    spindle assembly GO:0051225 ISO
    odontoblast differentiation GO:0071895 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nuclear envelope lumen GO:0005641 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    microtubule cytoskeleton GO:0015630 ISO
    motile cilium GO:0031514 IEA
    protein-containing complex GO:0032991 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    cell body GO:0044297 ISO
    membrane raft GO:0045121 IDA
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
 Experiment description of studies that identified Tubb5 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 30
MISEV standards
EM
Biophysical techniques
Alix|TSG101|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors "Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R"
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Tubb5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HDAC6 10013
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
2 PRICKLE2  
Affinity Capture-MS Homo sapiens
3 PRICKLE1  
Affinity Capture-MS Homo sapiens
4 Mib1  
Affinity Capture-MS Rattus norvegicus
5 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
6 Sumo3  
Affinity Capture-MS Rattus norvegicus
7 Park7 117287
Affinity Capture-MS Rattus norvegicus
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