Gene description for Mlc-c
Gene name Myosin light chain cytoplasmic
Gene symbol Mlc-c
Other names/aliases CG3201
DmMLC_C
DmelCG3201
MyoII
mlc-c
nmELC
Species Drosophila melanogaster
 Database cross references - Mlc-c
ExoCarta ExoCarta_31474
Vesiclepedia VP_31474
Entrez Gene 31474
UniProt P54357  
 Mlc-c identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Mlc-c
Molecular Function
    protein binding GO:0005515 IPI
    myosin binding GO:0017022 IPI
    myosin heavy chain binding GO:0032036 IPI
Biological Process
    actin filament-based movement GO:0030048 IC
    actin filament-based movement GO:0030048 IDA
Subcellular Localization
    myosin II complex GO:0016460 IPI
    myosin V complex GO:0031475 IPI
    myosin VI complex GO:0031476 IPI
    myosin VII complex GO:0031477 IPI
 Experiment description of studies that identified Mlc-c in sEVs
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Mlc-c
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CG11459  
Affinity Capture-MS Drosophila melanogaster
2 CG12691  
Affinity Capture-MS Drosophila melanogaster
3 Pglym87  
Affinity Capture-MS Drosophila melanogaster
4 CG13458  
Two-hybrid Drosophila melanogaster
5 His2B:CG17949  
Two-hybrid Drosophila melanogaster
6 CG11377  
Two-hybrid Drosophila melanogaster
7 Cpr100A  
Two-hybrid Drosophila melanogaster
8 CG32276  
Two-hybrid Drosophila melanogaster
9 cry  
Proximity Label-MS Drosophila melanogaster
10 Tm1 41852
Affinity Capture-MS Drosophila melanogaster
11 Ela  
Two-hybrid Drosophila melanogaster
12 CG10194  
Affinity Capture-MS Drosophila melanogaster
13 TFAM  
Two-hybrid Drosophila melanogaster
14 Fibp  
Affinity Capture-MS Drosophila melanogaster
15 CG15631  
Two-hybrid Drosophila melanogaster
16 CG1307  
Two-hybrid Drosophila melanogaster
17 CG14185  
Two-hybrid Drosophila melanogaster
18 zip 38001
Affinity Capture-MS Drosophila melanogaster
19 CG6412  
Two-hybrid Drosophila melanogaster
20 didum  
Two-hybrid Drosophila melanogaster
21 CG6664  
Two-hybrid Drosophila melanogaster
22 Pect  
Two-hybrid Drosophila melanogaster
23 CG14995  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml



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