Gene description for CG32549
Gene name CG32549 gene product from transcript CG32549-RD
Gene symbol CG32549
Other names/aliases CG6247
CG6267
CG6278
DmelCG32549
Species Drosophila melanogaster
 Database cross references - CG32549
ExoCarta ExoCarta_32822
Vesiclepedia VP_32822
Entrez Gene 32822
 CG32549 identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for CG32549
Subcellular Localization
    cytosol GO:0005829 NAS
 Experiment description of studies that identified CG32549 in sEVs
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CG32549
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Inos 35671
Affinity Capture-MS Drosophila melanogaster
2 Df31  
Affinity Capture-MS Drosophila melanogaster
3 Hsp26  
Affinity Capture-MS Drosophila melanogaster
4 CG6543  
Affinity Capture-MS Drosophila melanogaster
5 CG16817 41173
Affinity Capture-MS Drosophila melanogaster
6 Nacalpha  
Affinity Capture-MS Drosophila melanogaster
7 CG32549 32822
Two-hybrid Drosophila melanogaster
Two-hybrid Drosophila melanogaster
8 Su(var)3-9  
Two-hybrid Drosophila melanogaster
9 Hsp23  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which CG32549 is involved
No pathways found





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