Gene description for pnut
Gene name peanut
Gene symbol pnut
Other names/aliases CG8705
DmelCG8705
E(sina)44C
E(sina)8
PNUT
Pnut
anon-EST:Liang-2.18
clone 2.18
l(2)02502
Species Drosophila melanogaster
 Database cross references - pnut
ExoCarta ExoCarta_35801
Vesiclepedia VP_35801
Entrez Gene 35801
UniProt P40797  
 pnut identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for pnut
Molecular Function
    actin binding GO:0003779 IDA
    GTPase activity GO:0003924 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    microtubule binding GO:0008017 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    mitotic cytokinesis GO:0000281 IMP
    apoptotic process GO:0006915 IGI
    cellularization GO:0007349 TAS
    imaginal disc-derived wing morphogenesis GO:0007476 IMP
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of imaginal disc growth GO:0045572 IMP
Subcellular Localization
    plasma membrane GO:0005886 IDA
    septin complex GO:0031105 IDA
    septin complex GO:0031105 IPI
    cleavage furrow GO:0032154 IDA
    intercellular bridge GO:0045171 IDA
    contractile ring GO:0070938 IDA
 Experiment description of studies that identified pnut in exosomes
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for pnut
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CG9399  
Two-hybrid Drosophila melanogaster
2 CycK  
Two-hybrid Drosophila melanogaster
3 cry  
Proximity Label-MS Drosophila melanogaster
4 HIP-R 31335
Affinity Capture-MS Drosophila melanogaster
5 Sep2  
Affinity Capture-MS Drosophila melanogaster
6 STX1A 6804
Affinity Capture-Western Homo sapiens
7 Sep1 33114
Affinity Capture-MS Drosophila melanogaster
8 Sep5  
Affinity Capture-MS Drosophila melanogaster
9 park  
Affinity Capture-Western Drosophila melanogaster
View the network image/svg+xml
 Pathways in which pnut is involved
No pathways found





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