Gene description for pnut
Gene name peanut
Gene symbol pnut
Other names/aliases CG8705
DmelCG8705
E(sina)44C
E(sina)8
PNUT
Pnut
anon-EST:Liang-2.18
clone 2.18
l(2)02502
Species Drosophila melanogaster
 Database cross references - pnut
ExoCarta ExoCarta_35801
Entrez Gene 35801
UniProt P40797  
 pnut identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for pnut
Molecular Function
    actin binding GO:0003779 IDA
    GTP binding GO:0005525 IDA
    protein homodimerization activity GO:0042803 IDA
    microtubule binding GO:0008017 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    GTPase activity GO:0003924 IPI
Biological Process
    apoptotic process GO:0006915 IGI
    mitotic cytokinesis GO:0000281 ISS
    cellularization GO:0007349 TAS
    actomyosin contractile ring assembly GO:0000915 NAS
    positive regulation of apoptotic process GO:0043065 IDA
Subcellular Localization
    germline ring canal GO:0045172 NAS
    intercellular bridge GO:0045171 IDA
    cleavage furrow GO:0032154 IDA
    microtubule associated complex GO:0005875 IDA
    septin complex GO:0031105 IPI
    contractile ring GO:0070938 IDA
    mitochondrion GO:0005739 IDA
    septin ring GO:0005940 ISS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified pnut in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for pnut
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which pnut is involved
No pathways found





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