ExoCarta: Gene summary

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Gene description for RpS18
Gene name	Ribosomal protein S18
Gene symbol	RpS18
Other names/aliases	B BcDNA:RH43343 CG8900 DmelCG8900 RS18_DROME Rps18 S18 anon-56Fa anon-EST:Posey27 anon-EST:fe2D3 rpS18
Species	Drosophila melanogaster
Database cross references - RpS18
ExoCarta	ExoCarta_37292
Entrez Gene	37292
UniProt	P41094
RpS18 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells	23035643

Gene ontology annotations for RpS18

Molecular Function
	RNA binding	GO:0003723	IEA
	structural constituent of ribosome	GO:0003735	NAS
Biological Process
	translational initiation	GO:0006413	NAS
	mitotic spindle elongation	GO:0000022	IMP
	ribosome biogenesis	GO:0042254	IBA
	mitotic spindle organization	GO:0007052	IMP
	translation	GO:0006412	NAS
Subcellular Localization
	cytosol	GO:0005829	IBA
	nuclear chromosome	GO:0000228	IDA
	cytosolic small ribosomal subunit	GO:0022627	NAS
	cytoplasm	GO:0005737	IDA
	small ribosomal subunit	GO:0015935	IBA
	nucleolus	GO:0005730	IDA
	ribosome	GO:0005840	NAS

Experiment description of studies that identified RpS18 in exosomes

Experiment ID

202

ISEV standards
✔ IEM	EV Biophysical techniques
✔ TSG10\|RAB35	EV Cytosolic markers
✔ FLOT2	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23035643

Organism

Drosophila melanogaster

Experiment description

Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.

Authors

Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.

Journal name

Traffic

Publication year

2012

Sample

Drosophila S2 cells

Sample name

Normal-Drosophila S2 tub-Wg

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for RpS18

	Protein Interactor	ExoCarta ID	Identification method	PubMed	Species
No interactions are found.

Pathways in which RpS18 is involved

Pathway	Evidence	Source
Eukaryotic Translation Termination	IEA	Reactome
Formation of a pool of free 40S subunits	IEA	Reactome
Formation of the ternary complex, and subsequently, the 43S complex	IEA	Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit	IEA	Reactome
L13a-mediated translational silencing of Ceruloplasmin expression	IEA	Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)	IEA	Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)	IEA	Reactome
Peptide chain elongation	IEA	Reactome
Ribosomal scanning and start codon recognition	IEA	Reactome
SRP-dependent cotranslational protein targeting to membrane	IEA	Reactome
Translation initiation complex formation	IEA	Reactome

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