Gene description for MST1
Gene name macrophage stimulating 1
Gene symbol MST1
Other names/aliases D3F15S2
DNF15S2
HGFL
MSP
NF15S2
Species Homo sapiens
 Database cross references - MST1
ExoCarta ExoCarta_4485
Vesiclepedia VP_4485
Entrez Gene 4485
HGNC 7380
MIM 142408
UniProt P26927  
 MST1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MST1
Molecular Function
    endopeptidase activity GO:0004175 IBA
    protein binding GO:0005515 IPI
    receptor tyrosine kinase binding GO:0030971 IBA
    receptor tyrosine kinase binding GO:0030971 IDA
Biological Process
    proteolysis GO:0006508 IEA
    negative regulation of gluconeogenesis GO:0045721 IDA
    regulation of receptor signaling pathway via JAK-STAT GO:0046425 IBA
    regulation of cAMP-dependent protein kinase activity GO:2000479 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified MST1 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MST1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FOXO1  
Biochemical Activity Homo sapiens
2 RASSF1  
Two-hybrid Homo sapiens
3 LDLR 3949
Negative Genetic Homo sapiens
4 HSPA5 3309
Affinity Capture-MS Homo sapiens
5 BECN1 8678
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which MST1 is involved
PathwayEvidenceSource
Signal Transduction TAS Reactome
Signaling by MST1 TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome





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