Gene description for Nop58
Gene name NOP58 ribonucleoprotein
Gene symbol Nop58
Other names/aliases MSSP
Nol5
SIK
nop5
Species Mus musculus
 Database cross references - Nop58
ExoCarta ExoCarta_55989
Vesiclepedia VP_55989
Entrez Gene 55989
UniProt Q6DFW4  
 Nop58 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nop58
Molecular Function
    TFIID-class transcription factor complex binding GO:0001094 IEA
    TFIID-class transcription factor complex binding GO:0001094 ISO
    snoRNA binding GO:0030515 IBA
    snoRNA binding GO:0030515 IEA
    snoRNA binding GO:0030515 ISO
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
Biological Process
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
    snoRNA localization GO:0048254 IEA
    snoRNA localization GO:0048254 ISO
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    sno(s)RNA-containing ribonucleoprotein complex GO:0005732 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    Cajal body GO:0015030 IEA
    Cajal body GO:0015030 ISO
    box C/D methylation guide snoRNP complex GO:0031428 IBA
    box C/D methylation guide snoRNP complex GO:0031428 ISO
    box C/D methylation guide snoRNP complex GO:0031428 ISS
    small-subunit processome GO:0032040 IBA
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    pre-snoRNP complex GO:0070761 IEA
    pre-snoRNP complex GO:0070761 ISO
 Experiment description of studies that identified Nop58 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nop58
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mrto4  
Co-fractionation Mus musculus
2 Ftsj3  
Co-fractionation Mus musculus
3 Utp11l  
Co-fractionation Mus musculus
4 Eif5b 226982
Co-fractionation Mus musculus
5 Abt1  
Co-fractionation Mus musculus
6 Rrp1  
Co-fractionation Mus musculus
7 JADE1  
Affinity Capture-MS Homo sapiens
8 Nvl  
Co-fractionation Mus musculus
9 Ctps 51797
Co-fractionation Mus musculus
10 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
11 Riok1  
Co-fractionation Mus musculus
12 Cebpa  
Affinity Capture-MS Mus musculus
13 Wdr6  
Co-fractionation Mus musculus
14 Tfe3  
Affinity Capture-MS Mus musculus
15 Nop14  
Co-fractionation Mus musculus
16 Rrp12  
Co-fractionation Mus musculus
17 Mybbp1a 18432
Co-fractionation Mus musculus
18 Isg20l2  
Co-fractionation Mus musculus
19 Nufip1  
Co-fractionation Mus musculus
20 Utp14b  
Co-fractionation Mus musculus
21 Utp18  
Co-fractionation Mus musculus
22 Wdr43  
Co-fractionation Mus musculus
23 PRPF39 55015
Two-hybrid Homo sapiens
24 Fancd2  
Affinity Capture-MS Mus musculus
25 Polr1e  
Co-fractionation Mus musculus
26 Eed  
Affinity Capture-MS Mus musculus
27 Polr2e  
Co-fractionation Mus musculus
28 Aatf  
Co-fractionation Mus musculus
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