Gene description for Ywhag
Gene name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
Gene symbol Ywhag
Other names/aliases 14-3-3G
Species Rattus norvegicus
 Database cross references - Ywhag
ExoCarta ExoCarta_56010
Vesiclepedia VP_56010
Entrez Gene 56010
UniProt P61983  
 Ywhag identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ywhag
Molecular Function
    actin binding GO:0003779 ISS
    protein kinase C binding GO:0005080 IBA
    protein kinase C binding GO:0005080 ISO
    protein kinase C binding GO:0005080 ISS
    insulin-like growth factor receptor binding GO:0005159 ISO
    insulin-like growth factor receptor binding GO:0005159 ISS
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 ISO
    enzyme regulator activity GO:0030234 TAS
    receptor tyrosine kinase binding GO:0030971 IPI
    identical protein binding GO:0042802 ISO
    phosphorylation-dependent protein binding GO:0140031 ISO
    phosphorylation-dependent protein binding GO:0140031 ISS
    protein sequestering activity GO:0140311 ISO
    protein sequestering activity GO:0140311 ISS
Biological Process
    positive regulation of T cell mediated immune response to tumor cell GO:0002842 ISO
    protein targeting GO:0006605 ISO
    signal transduction GO:0007165 IBA
    protein localization GO:0008104 IBA
    positive regulation of cell-cell adhesion GO:0022409 ISO
    positive regulation of cell-cell adhesion GO:0022409 ISS
    cellular response to insulin stimulus GO:0032869 IDA
    regulation of protein localization GO:0032880 ISO
    regulation of protein localization GO:0032880 ISS
    cellular response to glucose starvation GO:0042149 ISO
    regulation of neuron differentiation GO:0045664 ISO
    regulation of neuron differentiation GO:0045664 ISS
    regulation of synaptic plasticity GO:0048167 ISO
    regulation of synaptic plasticity GO:0048167 ISS
    positive regulation of T cell activation GO:0050870 ISO
    negative regulation of TORC1 signaling GO:1904262 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISO
    vesicle GO:0031982 IDA
    synapse GO:0045202 IDA
    presynapse GO:0098793 IDA
 Experiment description of studies that identified Ywhag in exosomes
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ywhag
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Map3k3  
Two-hybrid Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
3 Park7 117287
Affinity Capture-MS Rattus norvegicus
4 Nedd4 25489
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Ywhag is involved
PathwayEvidenceSource
Activation of BAD and translocation to mitochondria IEA Reactome
Activation of BH3-only proteins IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
Apoptosis IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Centrosome maturation IEA Reactome
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex IEA Reactome
Cilium Assembly IEA Reactome
FOXO-mediated transcription IEA Reactome
G2/M Checkpoints IEA Reactome
G2/M DNA damage checkpoint IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Intrinsic Pathway for Apoptosis IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Prometaphase IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Programmed Cell Death IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of localization of FOXO transcription factors IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases activate PKNs IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
TP53 Regulates Metabolic Genes IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome





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