Gene description for Prkaa1
Gene name protein kinase, AMP-activated, alpha 1 catalytic subunit
Gene symbol Prkaa1
Other names/aliases AMPKalpha1
Species Rattus norvegicus
 Database cross references - Prkaa1
ExoCarta ExoCarta_65248
Entrez Gene 65248
UniProt P54645  
 Prkaa1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Prkaa1
Molecular Function
    chromatin binding GO:0003682 ISS
    kinase binding GO:0019900 IPI
    protein serine/threonine kinase activity GO:0004674 IDA
    AMP-activated protein kinase activity GO:0004679 ISO
    [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity GO:0047322 IEA
    tau-protein kinase activity GO:0050321 IEA
    eukaryotic elongation factor-2 kinase activator activity GO:0042557 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    protein C-terminus binding GO:0008022 IPI
    protein kinase activity GO:0004672 ISO
    metal ion binding GO:0046872 IEA
    histone serine kinase activity GO:0035174 ISS
    [acetyl-CoA carboxylase] kinase activity GO:0050405 IEA
Biological Process
    response to hydrogen peroxide GO:0042542 ISO
    rhythmic process GO:0048511 IEA
    regulation of energy homeostasis GO:2000505 IDA
    cellular response to hydrogen peroxide GO:0070301 IEP
    regulation of vesicle-mediated transport GO:0060627 IMP
    response to activity GO:0014823 IDA
    cellular response to ethanol GO:0071361 IEP
    negative regulation of glucose import in response to insulin stimulus GO:2001274 IMP
    regulation of circadian rhythm GO:0042752 ISS
    response to camptothecin GO:1901563 ISO
    negative regulation of lipid catabolic process GO:0050995 ISS
    regulation of transcription, DNA-templated GO:0006355 IEA
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of autophagy GO:0010508 ISS
    cold acclimation GO:0009631 IDA
    positive regulation of fatty acid oxidation GO:0046321 NAS
    regulation of peptidyl-serine phosphorylation GO:0033135 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    negative regulation of TOR signaling GO:0032007 ISS
    cellular response to nutrient levels GO:0031669 ISS
    cellular response to glucose starvation GO:0042149 ISS
    positive regulation of skeletal muscle tissue development GO:0048643 ISO
    fatty acid biosynthetic process GO:0006633 IEA
    response to UV GO:0009411 ISO
    activation of MAPK activity GO:0000187 IEA
    glucose homeostasis GO:0042593 ISS
    negative regulation of translation GO:0017148 NAS
    response to caffeine GO:0031000 IDA
    fatty acid homeostasis GO:0055089 IDA
    glucose metabolic process GO:0006006 ISO
    protein phosphorylation GO:0006468 NAS
    transcription, DNA-templated GO:0006351 IEA
    intracellular signal transduction GO:0035556 IBA
    positive regulation of gluconeogenesis GO:0045722 NAS
    positive regulation of cell proliferation GO:0008284 IDA
    lipid biosynthetic process GO:0008610 ISS
    cellular response to prostaglandin E stimulus GO:0071380 ISO
    Wnt signaling pathway GO:0016055 IEA
    response to gamma radiation GO:0010332 ISS
    histone-serine phosphorylation GO:0035404 ISO
    positive regulation of glucose import GO:0046326 NAS
    positive regulation of glycolytic process GO:0045821 IDA
    cellular response to organonitrogen compound GO:0071417 IEP
    cellular response to hypoxia GO:0071456 IEP
    fatty acid oxidation GO:0019395 ISO
    cholesterol biosynthetic process GO:0006695 IEA
    protein heterooligomerization GO:0051291 IDA
    autophagy GO:0006914 IEA
Subcellular Localization
    apical plasma membrane GO:0016324 IDA
    AMP-activated protein kinase complex GO:0031588 IDA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISO
    protein complex GO:0043234 IDA
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Prkaa1 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prkaa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFTR  
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here