Gene description for Eef1d
Gene name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Gene symbol Eef1d
Other names/aliases EF-1-delta
Species Mus musculus
 Database cross references - Eef1d
ExoCarta ExoCarta_66656
Entrez Gene 66656
UniProt P57776  
 Eef1d identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Pancreatic cells 19351151    
 Gene ontology annotations for Eef1d
Molecular Function
    heat shock protein binding GO:0031072 ISO
    signal transducer activity GO:0004871 ISO
    translation elongation factor activity GO:0003746 IEA
    activating transcription factor binding GO:0033613 ISO
    DNA binding GO:0003677 ISO
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    regulation of cell death GO:0010941 ISO
    regulation of transcription, DNA-templated GO:0006355 IEA
    cellular response to ionizing radiation GO:0071479 ISO
    translational elongation GO:0006414 IEA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISO
    signal transduction GO:0007165 ISO
    mRNA transcription GO:0009299 ISO
    translation GO:0006412 IEA
Subcellular Localization
    eukaryotic translation elongation factor 1 complex GO:0005853 IEA
    nucleolus GO:0005730 ISO
    nucleus GO:0005634 IDA
    endoplasmic reticulum GO:0005783 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Eef1d in exosomes
1
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Eef1d
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Eef1d is involved
PathwayEvidenceSource
Eukaryotic Translation Elongation IEA Reactome





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