Gene description for Cad
Gene name carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Gene symbol Cad
Other names/aliases 2410008J01Rik
AU018859
Cpad
Species Mus musculus
 Database cross references - Cad
ExoCarta ExoCarta_69719
Entrez Gene 69719
UniProt B2RQC6  
 Cad identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Cad
Molecular Function
    aspartate carbamoyltransferase activity GO:0004070 ISO
    amino acid binding GO:0016597 IEA
    hydrolase activity GO:0016787 IEA
    zinc ion binding GO:0008270 ISO
    carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity GO:0004088 ISO
    identical protein binding GO:0042802 ISO
    transferase activity GO:0016740 IEA
    catalytic activity GO:0003824 IEA
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds GO:0016810 IEA
    carboxyl- or carbamoyltransferase activity GO:0016743 IEA
    ATP binding GO:0005524 ISO
    dihydroorotase activity GO:0004151 ISO
    enzyme binding GO:0019899 ISO
    protein kinase activity GO:0004672 ISO
    metal ion binding GO:0046872 IEA
    ligase activity GO:0016874 IEA
    nucleotide binding GO:0000166 IEA
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides GO:0016812 IEA
    carbamoyl-phosphate synthase (ammonia) activity GO:0004087 IBA
    UTP binding GO:0002134 ISO
Biological Process
    protein autophosphorylation GO:0046777 ISO
    cellular amino acid metabolic process GO:0006520 IEA
    UTP biosynthetic process GO:0006228 ISO
    arginine biosynthetic process GO:0006526 IBA
    nitrogen compound metabolic process GO:0006807 IEA
    pyrimidine nucleotide biosynthetic process GO:0006221 IEA
    glutamine metabolic process GO:0006541 IEA
    metabolic process GO:0008152 IEA
    urea cycle GO:0000050 IBA
    peptidyl-threonine phosphorylation GO:0018107 ISO
    drug metabolic process GO:0017144 ISO
    'de novo' pyrimidine nucleobase biosynthetic process GO:0006207 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISO
    terminal bouton GO:0043195 ISO
    nuclear matrix GO:0016363 ISO
    protein complex GO:0043234 ISO
    plasma membrane GO:0005886 ISO
    cell projection GO:0042995 ISO
    neuronal cell body GO:0043025 ISO
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Cad in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Cad
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Cad is involved
PathwayEvidenceSource
Pyrimidine biosynthesis IEA Reactome





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