Gene description for CA12
Gene name carbonic anhydrase XII
Gene symbol CA12
Other names/aliases CAXII
HsT18816
Species Homo sapiens
 Database cross references - CA12
ExoCarta ExoCarta_771
Entrez Gene 771
HGNC 1371
MIM 603263
UniProt O43570  
 CA12 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CA12
Molecular Function
    zinc ion binding GO:0008270 IEA
    carbonate dehydratase activity GO:0004089 IEA
Biological Process
    chloride ion homeostasis GO:0055064 IMP
    one-carbon metabolic process GO:0006730 IEA
    small molecule metabolic process GO:0044281 TAS
    bicarbonate transport GO:0015701 TAS
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified CA12 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CA12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CA12 771
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
2 RUTBC1  
Two-hybrid Homo sapiens
3 ATP5C1 509
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CA12 is involved
PathwayEvidenceSource
Reversible hydration of carbon dioxide TAS Reactome





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