Gene description for FZD4
Gene name frizzled class receptor 4
Gene symbol FZD4
Other names/aliases CD344
EVR1
FEVR
FZD4S
Fz-4
Fz4
FzE4
GPCR
hFz4
Species Homo sapiens
 Database cross references - FZD4
ExoCarta ExoCarta_8322
Entrez Gene 8322
HGNC 4042
MIM 604579
UniProt Q9ULV1  
 FZD4 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for FZD4
Molecular Function
    PDZ domain binding GO:0030165 IPI
    protein heterodimerization activity GO:0046982 IPI
    protein binding GO:0005515 IPI
    G-protein coupled receptor activity GO:0004930 IBA
    protein homodimerization activity GO:0042803 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    Wnt-activated receptor activity GO:0042813 IDA
    Wnt-protein binding GO:0017147 IBA
    cytokine binding GO:0019955 IPI
Biological Process
    Wnt signaling pathway, calcium modulating pathway GO:0007223 IDA
    retina vasculature morphogenesis in camera-type eye GO:0061299 IMP
    cellular response to retinoic acid GO:0071300 ISS
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    canonical Wnt signaling pathway GO:0060070 IDA
    progesterone secretion GO:0042701 IEA
    sensory perception of sound GO:0007605 IEA
    vasculogenesis GO:0001570 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    Wnt signaling pathway GO:0016055 IDA
    cerebellum vasculature morphogenesis GO:0061301 IEA
    substrate adhesion-dependent cell spreading GO:0034446 IEA
    positive regulation of JUN kinase activity GO:0043507 IEA
    retinal blood vessel morphogenesis GO:0061304 IEA
    regulation of vascular endothelial growth factor receptor signaling pathway GO:0030947 IEA
    locomotion involved in locomotory behavior GO:0031987 IEA
    G-protein coupled receptor signaling pathway GO:0007186 IEA
    extracellular matrix-cell signaling GO:0035426 IEA
    negative regulation of cell-substrate adhesion GO:0010812 IMP
    neuron differentiation GO:0030182 ISS
Subcellular Localization
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    extracellular exosome GO:0070062 IDA
    integral component of membrane GO:0016021 IBA
    cell-cell junction GO:0005911 IEA
    integral component of plasma membrane GO:0005887 IC
 Experiment description of studies that identified FZD4 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for FZD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DLG4  
Invivo Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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