Gene description for Sdcbp
Gene name syndecan binding protein
Gene symbol Sdcbp
Other names/aliases TACIP18
mda-9
Species Rattus norvegicus
 Database cross references - Sdcbp
ExoCarta ExoCarta_83841
Vesiclepedia VP_83841
Entrez Gene 83841
UniProt Q9JI92  
 Sdcbp identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Sdcbp
Molecular Function
    frizzled binding GO:0005109 ISO
    interleukin-5 receptor binding GO:0005137 ISO
    interleukin-5 receptor binding GO:0005137 ISS
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISO
    growth factor binding GO:0019838 IPI
    ionotropic glutamate receptor binding GO:0035255 IPI
    neurexin family protein binding GO:0042043 IPI
    neurexin family protein binding GO:0042043 NAS
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 IPI
    syndecan binding GO:0045545 IBA
    syndecan binding GO:0045545 IPI
    syndecan binding GO:0045545 ISO
    ephrin receptor binding GO:0046875 IPI
    protein heterodimerization activity GO:0046982 ISO
    cell adhesion molecule binding GO:0050839 IPI
    protein sequestering activity GO:0140311 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    negative regulation of receptor internalization GO:0002091 ISO
    negative regulation of receptor internalization GO:0002091 ISS
    Ras protein signal transduction GO:0007265 ISO
    regulation of mitotic cell cycle GO:0007346 ISO
    regulation of mitotic cell cycle GO:0007346 ISS
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of cell population proliferation GO:0008284 ISS
    positive regulation of epithelial to mesenchymal transition GO:0010718 ISO
    positive regulation of epithelial to mesenchymal transition GO:0010718 ISS
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of cell growth GO:0030307 ISS
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of cell migration GO:0030335 ISS
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 IBA
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISO
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISS
    positive regulation of phosphorylation GO:0042327 ISO
    presynapse assembly GO:0099054 IDA
    presynapse assembly GO:0099054 IMP
    positive regulation of exosomal secretion GO:1903543 ISO
    positive regulation of extracellular exosome assembly GO:1903553 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    interleukin-5 receptor complex GO:0005895 ISO
    interleukin-5 receptor complex GO:0005895 ISS
    adherens junction GO:0005912 IEA
    focal adhesion GO:0005925 IEA
    membrane GO:0016020 IEA
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
    melanosome GO:0042470 IEA
    membrane raft GO:0045121 ISO
    membrane raft GO:0045121 ISS
    extracellular exosome GO:0070062 ISO
    presynapse GO:0098793 IDA
 Experiment description of studies that identified Sdcbp in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 99
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sdcbp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Grik1  
Two-hybrid Rattus norvegicus
2 Sdc2  
Two-hybrid Rattus norvegicus
3 Sdcbp2  
Two-hybrid Rattus norvegicus
Two-hybrid Rattus norvegicus
4 Sdcbp 83841
Two-hybrid Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Co-purification Rattus norvegicus
Two-hybrid Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Co-purification Rattus norvegicus
5 Igsf4  
Two-hybrid Rattus norvegicus
6 Gria2 29627
Two-hybrid Rattus norvegicus
7 Efnb2  
Two-hybrid Rattus norvegicus
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