Gene description for GALNT4
Gene name polypeptide N-acetylgalactosaminyltransferase 4
Gene symbol GALNT4
Other names/aliases GALNAC-T4
GALNACT4
Species Homo sapiens
 Database cross references - GALNT4
ExoCarta ExoCarta_8693
Vesiclepedia VP_8693
Entrez Gene 8693
HGNC 4126
MIM 603565
UniProt Q8N4A0  
 GALNT4 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
 Gene ontology annotations for GALNT4
Molecular Function
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IEA
    metal ion binding GO:0046872 IEA
    carbohydrate binding GO:0030246 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 NAS
    protein O-linked glycosylation GO:0006493 TAS
    post-translational protein modification GO:0043687 TAS
    cellular protein metabolic process GO:0044267 TAS
    O-glycan processing GO:0016266 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi membrane GO:0000139 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified GALNT4 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 66
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix|CD63|CD81
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for GALNT4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MUC1 4582
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which GALNT4 is involved
PathwayEvidenceSource
O-linked glycosylation of mucins TAS Reactome





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