Gene description for RGN
Gene name regucalcin
Gene symbol RGN
Other names/aliases GNL
HEL-S-41
RC
SMP30
Species Homo sapiens
 Database cross references - RGN
ExoCarta ExoCarta_9104
Vesiclepedia VP_9104
Entrez Gene 9104
HGNC 9989
MIM 300212
UniProt Q15493  
 RGN identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for RGN
Molecular Function
    gluconolactonase activity GO:0004341 IBA
    gluconolactonase activity GO:0004341 IDA
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    calcium ion binding GO:0005509 ISS
    zinc ion binding GO:0008270 IDA
    enzyme regulator activity GO:0030234 IEA
Biological Process
    kidney development GO:0001822 IEA
    intracellular calcium ion homeostasis GO:0006874 ISS
    spermatogenesis GO:0007283 IEA
    positive regulation of triglyceride biosynthetic process GO:0010867 IEA
    positive regulation of glucose metabolic process GO:0010907 IEA
    positive regulation of ATP-dependent activity GO:0032781 ISS
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of nitric oxide biosynthetic process GO:0045019 IEA
    positive regulation of fatty acid biosynthetic process GO:0045723 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    regulation of calcium-mediated signaling GO:0050848 ISS
    liver regeneration GO:0097421 IEA
    negative regulation of flagellated sperm motility GO:1901318 IEA
    negative regulation of RNA biosynthetic process GO:1902679 IEA
    negative regulation of bone development GO:1903011 IEA
    positive regulation of proteolysis involved in protein catabolic process GO:1903052 IEA
    negative regulation of DNA catabolic process GO:1903625 IEA
    negative regulation of DNA biosynthetic process GO:2000279 IEA
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified RGN in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for RGN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 THRA  
Two-hybrid Homo sapiens
2 HSPE1 3336
Co-fractionation Homo sapiens
3 OAZ3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SGOL2 151246
Affinity Capture-MS Homo sapiens
5 SOD1 6647
Co-fractionation Homo sapiens
6 HINT1 3094
Co-fractionation Homo sapiens
7 WDR1 9948
Co-fractionation Homo sapiens
8 CTNNB1 1499
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which RGN is involved
No pathways found





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