Gene description for CD86
Gene name CD86 molecule
Gene symbol CD86
Other names/aliases B7-2
B7.2
B70
CD28LG2
LAB72
Species Homo sapiens
 Database cross references - CD86
ExoCarta ExoCarta_942
Entrez Gene 942
HGNC 1705
MIM 601020
UniProt P42081  
 CD86 identified in exosomes derived from the following tissue/cell type
B cell lymphoblastoid cells 10878338    
B cells 9685355    
B cells 17641064    
Breast milk 17641064    
Breast milk 17641064    
Bronchoalveolar lavage fluid 14582906    
Dendritic cells 14582906    
 Gene ontology annotations for CD86
Molecular Function
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 NAS
    receptor activity GO:0004872 TAS
    receptor binding GO:0005102 IEA
Biological Process
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    response to interferon-gamma GO:0034341 IEA
    response to drug GO:0042493 IEA
    innate immune response GO:0045087 TAS
    positive regulation of interleukin-2 biosynthetic process GO:0045086 NAS
    positive regulation of interleukin-4 biosynthetic process GO:0045404 NAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    cell-cell signaling GO:0007267 IC
    positive regulation of T-helper 2 cell differentiation GO:0045630 NAS
    toll-like receptor signaling pathway GO:0002224 IEA
    T cell proliferation involved in immune response GO:0002309 IEA
    positive regulation of transcription, DNA-templated GO:0045893 NAS
    immune response GO:0006955 TAS
    defense response to virus GO:0051607 IEA
    B cell activation GO:0042113 IEA
    T cell activation GO:0042110 IC
    T cell costimulation GO:0031295 TAS
    cellular response to cytokine stimulus GO:0071345 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    positive regulation of lymphotoxin A biosynthetic process GO:0043017 NAS
    positive regulation of cell proliferation GO:0008284 TAS
    cellular response to lipopolysaccharide GO:0071222 IEA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    positive regulation of activated T cell proliferation GO:0042104 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    cellular response to metal ion GO:0071248 IEA
    negative regulation of T cell anergy GO:0002668 IEA
    viral process GO:0016032 IEA
    response to yeast GO:0001878 IEA
    myeloid dendritic cell differentiation GO:0043011 IEA
    aging GO:0007568 IEA
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IEA
    external side of plasma membrane GO:0009897 IEA
    cell surface GO:0009986 IDA
    integral component of membrane GO:0016021 IC
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified CD86 in exosomes
1
Experiment ID 37
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD86
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 10878338    
Organism Homo sapiens
Experiment description Direct immunosuppressive effects of EBV-encoded latent membrane protein 1.
Authors Dukers DF, Meij P, Vervoort MB, Vos W, Scheper RJ, Meijer CJ, Bloemena E, Middeldorp JM
Journal name JIMMU
Publication year 2000
Sample B cell lymphoblastoid cells
Sample name JY
B95-8
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 16
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 9685355    
Organism Homo sapiens
Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ
Journal name JBC
Publication year 1998
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.12-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
3
Experiment ID 47
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|CD86
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample B cells
Sample name B cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
4
Experiment ID 46
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|MHCII|CD81|CD86
EV Membrane markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
5
Experiment ID 48
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|CD86|MHCII
EV Membrane markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
6
Experiment ID 49
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD86
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 14582906    
Organism Homo sapiens
Experiment description Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name ERJ
Publication year 2003
Sample Bronchoalveolar lavage fluid
Sample name Bronchoalveolar lavage fluid
Isolation/purification methods Differential centrifugation
Immunobeads (EpCAM)
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
7
Experiment ID 50
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD87
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 14582906    
Organism Homo sapiens
Experiment description Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name ERJ
Publication year 2003
Sample Dendritic cells
Sample name Monocyte-derived dendritic cells
Isolation/purification methods Differential centrifugation
Immunobeads (EpCAM)
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
 Protein-protein interactions for CD86
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD86 942
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CTLA4  
Co-crystal Structure Homo sapiens
View the network image/svg+xml



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