 Experiment description of studies that identified CD86 in sEVs |
| 1 |
| Experiment ID |
37 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD86
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
10878338
|
| Organism |
Homo sapiens |
| Experiment description |
Direct immunosuppressive effects of EBV-encoded latent membrane protein 1. |
| Authors |
"Dukers DF, Meij P, Vervoort MB, Vos W, Scheper RJ, Meijer CJ, Bloemena E, Middeldorp JM" |
| Journal name |
JIMMU
|
| Publication year |
2000 |
| Sample |
B cell lymphoblastoid cells |
| Sample name |
JY
B95-8 |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 2 |
| Experiment ID |
16 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
9685355
|
| Organism |
Homo sapiens |
| Experiment description |
Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. |
| Authors |
"Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ" |
| Journal name |
JBC
|
| Publication year |
1998 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15+) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.12-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunoelectron Microscopy |
|
|
| 3 |
| Experiment ID |
47 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|CD86
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
FACS
|
| PubMed ID |
17641064
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with immune modulatory features are present in human breast milk. |
| Authors |
"Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S" |
| Journal name |
JIMMU
|
| Publication year |
2007 |
| Sample |
B cells |
| Sample name |
B cells |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
FACS |
|
|
| 4 |
| Experiment ID |
46 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
HSC70|CD63|MHCII|CD81|CD86
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
FACS
|
| PubMed ID |
17641064
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with immune modulatory features are present in human breast milk. |
| Authors |
"Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S" |
| Journal name |
JIMMU
|
| Publication year |
2007 |
| Sample |
Breast milk |
| Sample name |
Breast milk - Colostrum |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sucrose density gradient |
| Flotation density |
1.10-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS |
|
|
| 5 |
| Experiment ID |
48 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
HSC70|CD63|CD86|MHCII
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
FACS
|
| PubMed ID |
17641064
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with immune modulatory features are present in human breast milk. |
| Authors |
"Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S" |
| Journal name |
JIMMU
|
| Publication year |
2007 |
| Sample |
Breast milk |
| Sample name |
Breast milk - Mature milk |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sucrose density gradient |
| Flotation density |
1.10-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS |
|
|
| 6 |
| Experiment ID |
49 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
CD63|CD86
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
FACS
|
| PubMed ID |
14582906
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid. |
| Authors |
"Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S" |
| Journal name |
ERJ
|
| Publication year |
2003 |
| Sample |
Bronchoalveolar lavage fluid |
| Sample name |
Bronchoalveolar lavage fluid |
| Isolation/purification methods |
Differential centrifugation
Immunobeads (EpCAM) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
FACS
Immunoelectron Microscopy |
|
|
| 7 |
| Experiment ID |
50 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
CD63|CD87
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
FACS
|
| PubMed ID |
14582906
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid. |
| Authors |
"Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S" |
| Journal name |
ERJ
|
| Publication year |
2003 |
| Sample |
Dendritic cells |
| Sample name |
Monocyte-derived dendritic cells |
| Isolation/purification methods |
Differential centrifugation
Immunobeads (EpCAM) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
FACS
Immunoelectron Microscopy |
|
|
