Gene description for LIMS3
Gene name LIM and senescent cell antigen-like domains 3
Gene symbol LIMS3
Other names/aliases PINCH-3
Species Homo sapiens
 Database cross references - LIMS3
ExoCarta ExoCarta_96626
Vesiclepedia VP_96626
Entrez Gene 96626
HGNC 30047
UniProt P0CW20   P0CW19  
 LIMS3 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LIMS3
Molecular Function
    metal ion binding GO:0046872 IEA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    focal adhesion GO:0005925 IEA
 Experiment description of studies that identified LIMS3 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LIMS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BTF3 689
Affinity Capture-MS Homo sapiens
2 CRX  
Two-hybrid Homo sapiens
3 BCL6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 FAM219A 203259
Two-hybrid Homo sapiens
5 GMCL1P1  
Two-hybrid Homo sapiens
6 TRIM49C  
Two-hybrid Homo sapiens
7 KRTAP11-1  
Two-hybrid Homo sapiens
8 C10orf55  
Two-hybrid Homo sapiens
9 RUSC1  
Two-hybrid Homo sapiens
10 ARID5A  
Two-hybrid Homo sapiens
11 KLHDC7B  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which LIMS3 is involved
No pathways found





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