Gene description for Hmgb2
Gene name high mobility group box 2
Gene symbol Hmgb2
Other names/aliases C80539
HMG-2
Hmg2
Species Mus musculus
 Database cross references - Hmgb2
ExoCarta ExoCarta_97165
Vesiclepedia VP_97165
Entrez Gene 97165
UniProt P30681  
 Hmgb2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hmgb2
Molecular Function
    DNA binding, bending GO:0008301 ISO
    transcription regulatory region DNA binding GO:0044212 ISO
    protein domain specific binding GO:0019904 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 ISO
    DNA binding GO:0003677 ISO
    chemoattractant activity GO:0042056 ISO
    RAGE receptor binding GO:0050786 ISO
    poly(A) RNA binding GO:0044822 ISO
    damaged DNA binding GO:0003684 ISO
    chromatin binding GO:0003682 IBA
Biological Process
    spermatid nucleus differentiation GO:0007289 IMP
    positive regulation of endothelial cell proliferation GO:0001938 ISO
    positive regulation of transcription, DNA-templated GO:0045893 ISO
    positive regulation of DNA binding GO:0043388 ISO
    response to steroid hormone GO:0048545 IMP
    base-excision repair, DNA ligation GO:0006288 ISO
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IMP
    cell chemotaxis GO:0060326 ISO
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    male gonad development GO:0008584 IMP
    positive regulation of erythrocyte differentiation GO:0045648 ISO
    positive regulation of nuclease activity GO:0032075 ISO
    spermatogenesis GO:0007283 IMP
    regulation of transcription from RNA polymerase II promoter GO:0006357 ISO
    positive regulation of megakaryocyte differentiation GO:0045654 ISO
    positive chemotaxis GO:0050918 ISO
    chromatin remodeling GO:0006338 IBA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 ISO
    chromosome GO:0005694 IEA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 ISO
    condensed chromosome GO:0000793 ISO
    protein complex GO:0043234 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Hmgb2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hmgb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hmgb2 is involved
PathwayEvidenceSource
Activation of DNA fragmentation factor IEA Reactome





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