Gene description for Hmgb2
Gene name high mobility group box 2
Gene symbol Hmgb2
Other names/aliases C80539
HMG-2
Hmg2
Species Mus musculus
 Database cross references - Hmgb2
ExoCarta ExoCarta_97165
Vesiclepedia VP_97165
Entrez Gene 97165
UniProt P30681  
 Hmgb2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hmgb2
Molecular Function
    transcription cis-regulatory region binding GO:0000976 ISO
    cis-regulatory region sequence-specific DNA binding GO:0000987 IDA
    DNA binding GO:0003677 ISO
    DNA binding GO:0003677 ISS
    damaged DNA binding GO:0003684 IEA
    damaged DNA binding GO:0003684 ISO
    double-stranded DNA binding GO:0003690 IDA
    double-stranded DNA binding GO:0003690 ISS
    transcription coactivator activity GO:0003713 IEA
    transcription coactivator activity GO:0003713 ISO
    protein binding GO:0005515 IPI
    transcription factor binding GO:0008134 IDA
    lipid binding GO:0008289 ISO
    DNA binding, bending GO:0008301 IBA
    DNA binding, bending GO:0008301 ISO
    DNA binding, bending GO:0008301 ISS
    protein domain specific binding GO:0019904 IPI
    chemoattractant activity GO:0042056 IEA
    chemoattractant activity GO:0042056 ISO
    non-sequence-specific DNA binding, bending GO:0044378 ISS
    RAGE receptor binding GO:0050786 IEA
    RAGE receptor binding GO:0050786 ISO
    supercoiled DNA binding GO:0097100 ISS
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    positive regulation of endothelial cell proliferation GO:0001938 IEA
    positive regulation of endothelial cell proliferation GO:0001938 ISO
    inflammatory response to antigenic stimulus GO:0002437 IMP
    DNA topological change GO:0006265 ISS
    DNA recombination GO:0006310 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    regulation of transcription by RNA polymerase II GO:0006357 ISS
    spermatogenesis GO:0007283 IMP
    spermatid nucleus differentiation GO:0007289 IMP
    male gonad development GO:0008584 IMP
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IMP
    positive regulation of gene expression GO:0010628 IDA
    negative regulation of gene expression GO:0010629 IDA
    response to lipopolysaccharide GO:0032496 IMP
    positive regulation of interferon-beta production GO:0032728 IMP
    positive regulation of DNA binding GO:0043388 IDA
    innate immune response GO:0045087 IEA
    positive regulation of innate immune response GO:0045089 IMP
    positive regulation of erythrocyte differentiation GO:0045648 IEA
    positive regulation of erythrocyte differentiation GO:0045648 ISO
    positive regulation of megakaryocyte differentiation GO:0045654 IEA
    positive regulation of megakaryocyte differentiation GO:0045654 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    response to steroid hormone GO:0048545 IMP
    regulation of neurogenesis GO:0050767 IMP
    defense response to Gram-negative bacterium GO:0050829 ISO
    defense response to Gram-negative bacterium GO:0050829 ISS
    defense response to Gram-positive bacterium GO:0050830 ISO
    defense response to Gram-positive bacterium GO:0050830 ISS
    positive chemotaxis GO:0050918 IEA
    cell chemotaxis GO:0060326 IEA
    cell chemotaxis GO:0060326 ISO
    cellular response to lipopolysaccharide GO:0071222 IEA
    regulation of stem cell proliferation GO:0072091 IMP
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IMP
Subcellular Localization
    chromatin GO:0000785 IDA
    condensed chromosome GO:0000793 ISO
    condensed chromosome GO:0000793 ISS
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 IC
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
 Experiment description of studies that identified Hmgb2 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hmgb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 Lef1 16842
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
3 Atg5  
Affinity Capture-RNA Mus musculus
4 Ctnnb1 12387
Reconstituted Complex Mus musculus
5 VASP 7408
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Hmgb2 is involved
PathwayEvidenceSource
Apoptosis IEA Reactome
Apoptosis induced DNA fragmentation IEA Reactome
Apoptotic execution phase IEA Reactome
Programmed Cell Death IEA Reactome





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