Experiment description of studies that identified Ehd4 in exosomes

1 Experiment ID 214 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors Hassani K, Olivier M. Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name Leishmania-infected-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 215 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors Hassani K, Olivier M. Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name LPS-treated-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 216 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors Hassani K, Olivier M. Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name Normal-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 15 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

Protein-protein interactions for Ehd4

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Ehd4 is involved

No pathways found