Gene description for PTGS2
Gene name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Gene symbol PTGS2
Other names/aliases COX-2
COX2
GRIPGHS
PGG/HS
PGHS-2
PHS-2
hCox-2
Species Homo sapiens
 Database cross references - PTGS2
ExoCarta ExoCarta_5743
Vesiclepedia VP_5743
Entrez Gene 5743
HGNC 9605
MIM 600262
UniProt P35354  
 PTGS2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PTGS2
Molecular Function
    peroxidase activity GO:0004601 NAS
    prostaglandin-endoperoxide synthase activity GO:0004666 IBA
    prostaglandin-endoperoxide synthase activity GO:0004666 IDA
    protein binding GO:0005515 IPI
    oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen GO:0016702 IBA
    enzyme binding GO:0019899 IPI
    heme binding GO:0020037 ISS
    protein homodimerization activity GO:0042803 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    prostaglandin biosynthetic process GO:0001516 IDA
    prostaglandin biosynthetic process GO:0001516 ISS
    prostaglandin biosynthetic process GO:0001516 NAS
    angiogenesis GO:0001525 IEA
    response to oxidative stress GO:0006979 IEA
    embryo implantation GO:0007566 IEA
    learning GO:0007612 IEA
    memory GO:0007613 IEA
    regulation of blood pressure GO:0008217 ISS
    negative regulation of cell population proliferation GO:0008285 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to nematode GO:0009624 IEA
    response to fructose GO:0009750 IEA
    response to manganese ion GO:0010042 IEA
    response to selenium ion GO:0010269 IEA
    positive regulation of vascular endothelial growth factor production GO:0010575 ISS
    cyclooxygenase pathway GO:0019371 IBA
    cyclooxygenase pathway GO:0019371 IDA
    bone mineralization GO:0030282 IEA
    positive regulation of prostaglandin biosynthetic process GO:0031394 NAS
    positive regulation of fever generation GO:0031622 ISS
    positive regulation of synaptic plasticity GO:0031915 IEA
    negative regulation of synaptic transmission, dopaminergic GO:0032227 IEA
    prostaglandin secretion GO:0032310 IEA
    response to estradiol GO:0032355 IEA
    response to vitamin D GO:0033280 IEA
    cellular response to heat GO:0034605 IEA
    response to tumor necrosis factor GO:0034612 IEA
    maintenance of blood-brain barrier GO:0035633 IEA
    positive regulation of protein import into nucleus GO:0042307 IEA
    hair cycle GO:0042633 IEA
    positive regulation of apoptotic process GO:0043065 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISS
    negative regulation of cell cycle GO:0045786 IEA
    positive regulation of vasoconstriction GO:0045907 IEA
    negative regulation of smooth muscle contraction GO:0045986 IEA
    positive regulation of smooth muscle contraction GO:0045987 IEA
    decidualization GO:0046697 IEA
    positive regulation of smooth muscle cell proliferation GO:0048661 IEA
    regulation of inflammatory response GO:0050727 NAS
    brown fat cell differentiation GO:0050873 IEA
    response to glucocorticoid GO:0051384 IEA
    negative regulation of calcium ion transport GO:0051926 IEA
    positive regulation of synaptic transmission, glutamatergic GO:0051968 IEA
    response to fatty acid GO:0070542 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to lead ion GO:0071284 IEA
    cellular response to ATP GO:0071318 IEA
    cellular response to hypoxia GO:0071456 IEP
    cellular response to non-ionic osmotic stress GO:0071471 IEA
    cellular response to fluid shear stress GO:0071498 IEA
    positive regulation of transforming growth factor beta production GO:0071636 ISS
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 ISS
    positive regulation of fibroblast growth factor production GO:0090271 ISS
    positive regulation of brown fat cell differentiation GO:0090336 ISS
    positive regulation of platelet-derived growth factor production GO:0090362 ISS
    cellular oxidant detoxification GO:0098869 IEA
    regulation of neuroinflammatory response GO:0150077 ISS
    negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress GO:1902219 IEA
    cellular response to homocysteine GO:1905375 IEA
    response to angiotensin GO:1990776 IEA
Subcellular Localization
    nuclear inner membrane GO:0005637 IDA
    nuclear outer membrane GO:0005640 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
    caveola GO:0005901 IEA
    protein-containing complex GO:0032991 IEA
    neuron projection GO:0043005 IBA
    neuron projection GO:0043005 IDA
 Experiment description of studies that identified PTGS2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PTGS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPD 3184
Affinity Capture-RNA Homo sapiens
2 CUL1 8454
Affinity Capture-Western Homo sapiens
3 FAF2 23197
Affinity Capture-MS Homo sapiens
4 TP53 7157
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 COPS7A 50813
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 ELMO1 9844
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 CASK 8573
Two-hybrid Homo sapiens
8 USP22 23326
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 NFKB1 4790
Positive Genetic Homo sapiens
10 CTNNB1 1499
Protein-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
11 MAPK3 5595
Affinity Capture-Western Homo sapiens
12 SKP2  
Affinity Capture-MS Homo sapiens
13 LMAN2L 81562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PTGS1 5742
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 RBX1 9978
Affinity Capture-Western Homo sapiens
16 Vcp 269523
Affinity Capture-Western Mus musculus
17 ELAVL1 1994
Protein-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
Protein-RNA Homo sapiens
18 PTGS2 5743
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
19 AGTR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 CELF2 10659
Protein-RNA Homo sapiens
21 APP 351
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 HSPA4 3308
Affinity Capture-RNA Homo sapiens
23 PTGIS 5740
FRET Homo sapiens
24 NUCB1 4924
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
25 COPS5 10987
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
26 PSMC5 5705
Affinity Capture-Western Homo sapiens
27 SRC 6714
Positive Genetic Homo sapiens
28 COPS3 8533
Affinity Capture-Western Homo sapiens
29 PSMD1 5707
Affinity Capture-Western Homo sapiens
30 Drosha  
Affinity Capture-Western Mus musculus
31 DERL1 79139
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 VIMP 55829
Affinity Capture-Western Homo sapiens
33 Nos2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
34 CAV1 857
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
35 CUL4A 8451
Affinity Capture-Western Homo sapiens
36 EP300 2033
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 SUMO1 7341
Affinity Capture-Western Homo sapiens
38 PTGER1  
Affinity Capture-Western Homo sapiens
39 VCP 7415
Affinity Capture-Western Homo sapiens
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