Gene description for DENND4A
Gene name DENN/MADD domain containing 4A
Gene symbol DENND4A
Other names/aliases IRLB
MYCPBP
Species Homo sapiens
 Database cross references - DENND4A
ExoCarta ExoCarta_10260
Vesiclepedia VP_10260
Entrez Gene 10260
HGNC 24321
MIM 600382
UniProt Q7Z401  
 DENND4A identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for DENND4A
Molecular Function
    DNA binding GO:0003677 NAS
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
Biological Process
    regulation of DNA-templated transcription GO:0006355 NAS
    regulation of Rab protein signal transduction GO:0032483 IBA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    cytoplasmic vesicle GO:0031410 IBA
 Experiment description of studies that identified DENND4A in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DENND4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 DENND4C 55667
Affinity Capture-MS Homo sapiens
3 LRFN1  
Affinity Capture-MS Homo sapiens
4 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
5 VPS35 55737
Affinity Capture-MS Homo sapiens
6 LIMA1 51474
Affinity Capture-MS Homo sapiens
7 RAB35 11021
Proximity Label-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 SCRN1 9805
Affinity Capture-MS Homo sapiens
10 VPS29 51699
Affinity Capture-MS Homo sapiens
11 S100P 6286
Affinity Capture-MS Homo sapiens
12 DCLK1 9201
Affinity Capture-MS Homo sapiens
13 KSR1  
Affinity Capture-MS Homo sapiens
14 LAMTOR1 55004
Proximity Label-MS Homo sapiens
15 ATG9A 79065
Proximity Label-MS Homo sapiens
16 RTKN 6242
Affinity Capture-MS Homo sapiens
17 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 DENND1A 57706
Affinity Capture-MS Homo sapiens
19 PPM1H  
Affinity Capture-MS Homo sapiens
20 CALM2 805
Affinity Capture-MS Homo sapiens
21 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 NF1 4763
Affinity Capture-MS Homo sapiens
23 PTPRA 5786
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 S100A2 6273
Affinity Capture-MS Homo sapiens
25 SRGAP2 23380
Affinity Capture-MS Homo sapiens
26 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TLX3  
Two-hybrid Homo sapiens
28 MAP4K4 9448
Affinity Capture-MS Homo sapiens
29 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
30 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CBY1  
Affinity Capture-MS Homo sapiens
32 LYPD4  
Affinity Capture-MS Homo sapiens
33 ZBTB21  
Affinity Capture-MS Homo sapiens
34 DDX58 23586
Affinity Capture-RNA Homo sapiens
35 ASB3 51130
Affinity Capture-MS Homo sapiens
36 TESK2  
Affinity Capture-MS Homo sapiens
37 METTL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 EIF4E2  
Affinity Capture-MS Homo sapiens
39 RAB4A 5867
Proximity Label-MS Homo sapiens
40 KIF13B 23303
Affinity Capture-MS Homo sapiens
41 CEBPE  
Two-hybrid Homo sapiens
42 CGN  
Affinity Capture-MS Homo sapiens
43 NTRK3 4916
Affinity Capture-MS Homo sapiens
44 SERTAD1  
Two-hybrid Homo sapiens
45 KRT8 3856
Proximity Label-MS Homo sapiens
46 RPS13 6207
Cross-Linking-MS (XL-MS) Homo sapiens
47 PSMA7 5688
Cross-Linking-MS (XL-MS) Homo sapiens
48 KCTD3  
Affinity Capture-MS Homo sapiens
49 GIGYF1  
Affinity Capture-MS Homo sapiens
50 RAB7A 7879
Proximity Label-MS Homo sapiens
51 RAB9A 9367
Proximity Label-MS Homo sapiens
52 ZNF638 27332
Affinity Capture-MS Homo sapiens
53 MAGI1  
Affinity Capture-MS Homo sapiens
54 PCBD1 5092
Two-hybrid Homo sapiens
55 HSD17B7P2  
Affinity Capture-MS Homo sapiens
56 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SRSF12  
Affinity Capture-MS Homo sapiens
58 FLII 2314
Affinity Capture-MS Homo sapiens
59 CALM3 808
Affinity Capture-MS Homo sapiens
60 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 MAST3  
Affinity Capture-MS Homo sapiens
62 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
63 INPP5E 56623
Affinity Capture-MS Homo sapiens
64 HDAC4  
Affinity Capture-MS Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 CAV1 857
Proximity Label-MS Homo sapiens
67 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
68 UBA1 7317
Affinity Capture-MS Homo sapiens
69 STX6 10228
Proximity Label-MS Homo sapiens
70 RAB11A 8766
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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