Gene description for NISCH
Gene name nischarin
Gene symbol NISCH
Other names/aliases I-1
IR1
IRAS
hIRAS
Species Homo sapiens
 Database cross references - NISCH
ExoCarta ExoCarta_11188
Vesiclepedia VP_11188
Entrez Gene 11188
HGNC 18006
MIM 615507
UniProt Q9Y2I1  
 NISCH identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for NISCH
Molecular Function
    integrin binding GO:0005178 IEA
    protein binding GO:0005515 IPI
    phosphatidylinositol binding GO:0035091 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    apoptotic process GO:0006915 IEA
    Rac protein signal transduction GO:0016601 IEA
    actin cytoskeleton organization GO:0030036 IEA
    negative regulation of cell migration GO:0030336 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    microtubule cytoskeleton GO:0015630 IDA
    membrane GO:0016020 HDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    intercellular bridge GO:0045171 IDA
    recycling endosome GO:0055037 IEA
 Experiment description of studies that identified NISCH in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for NISCH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 Pik3r2  
Affinity Capture-MS Mus musculus
3 PCGF5  
Affinity Capture-MS Homo sapiens
4 SPRTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PER1  
Affinity Capture-MS Homo sapiens
6 LGR4 55366
Affinity Capture-MS Homo sapiens
7 OCLN 100506658
Proximity Label-MS Homo sapiens
8 LAMP3  
Proximity Label-MS Homo sapiens
9 RGS2  
Affinity Capture-MS Homo sapiens
10 GJA1 2697
Proximity Label-MS Homo sapiens
11 UBE2M 9040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CSNK1G2 1455
Affinity Capture-MS Homo sapiens
13 LAMP2 3920
Proximity Label-MS Homo sapiens
14 VHL  
Two-hybrid Homo sapiens
15 IGFBP4 3487
Affinity Capture-MS Homo sapiens
16 FAM219B 57184
Affinity Capture-MS Homo sapiens
17 ZNF414  
Affinity Capture-MS Homo sapiens
18 EIF4ENIF1 56478
Affinity Capture-MS Homo sapiens
19 ERF  
Affinity Capture-MS Homo sapiens
20 SENP3 26168
Affinity Capture-Western Homo sapiens
21 HELZ 9931
Affinity Capture-MS Homo sapiens
22 SLC31A1 1317
Affinity Capture-MS Homo sapiens
23 DDX58 23586
Affinity Capture-RNA Homo sapiens
24 SRP14 6727
Affinity Capture-MS Homo sapiens
25 PNOC  
Affinity Capture-MS Homo sapiens
26 PSMC1 5700
Affinity Capture-MS Homo sapiens
27 RAB35 11021
Proximity Label-MS Homo sapiens
28 LAMTOR1 55004
Proximity Label-MS Homo sapiens
29 PCGF3  
Affinity Capture-MS Homo sapiens
30 IRS4 8471
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
31 MANSC1 54682
Affinity Capture-MS Homo sapiens
32 ZNF444  
Affinity Capture-MS Homo sapiens
33 ATP6V1G2  
Affinity Capture-MS Homo sapiens
34 CTBP1 1487
Affinity Capture-MS Homo sapiens
35 CXADR 1525
Proximity Label-MS Homo sapiens
36 LYN 4067
Proximity Label-MS Homo sapiens
37 RAB4A 5867
Proximity Label-MS Homo sapiens
38 PAN2  
Affinity Capture-MS Homo sapiens
39 DNAJC5 80331
Proximity Label-MS Homo sapiens
40 GID8 54994
Affinity Capture-MS Homo sapiens
41 YWHAE 7531
Affinity Capture-MS Homo sapiens
42 DIRAS3  
Proximity Label-MS Homo sapiens
43 OGT 8473
Reconstituted Complex Homo sapiens
44 UNC119 9094
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 SOWAHA  
Affinity Capture-MS Homo sapiens
46 SND1 27044
Cross-Linking-MS (XL-MS) Homo sapiens
47 LEAP2  
Affinity Capture-MS Homo sapiens
48 ZNF747  
Affinity Capture-MS Homo sapiens
49 FOXH1  
Affinity Capture-MS Homo sapiens
50 C1orf111  
Affinity Capture-MS Homo sapiens
51 ABT1 29777
Affinity Capture-MS Homo sapiens
52 RAB5A 5868
Proximity Label-MS Homo sapiens
53 LAMP1 3916
Proximity Label-MS Homo sapiens
54 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 YWHAZ 7534
Affinity Capture-MS Homo sapiens
56 LOC254896  
Affinity Capture-MS Homo sapiens
57 ARF6 382
Proximity Label-MS Homo sapiens
58 ODAM  
Affinity Capture-MS Homo sapiens
59 CNKSR3 154043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 KIAA0430  
Affinity Capture-MS Homo sapiens
61 KCNE3  
Affinity Capture-MS Homo sapiens
62 EPHA2 1969
Proximity Label-MS Homo sapiens
63 ARID3A  
Affinity Capture-MS Homo sapiens
64 RPA3 6119
Proximity Label-MS Homo sapiens
65 RAB9A 9367
Proximity Label-MS Homo sapiens
66 KIAA1671  
Affinity Capture-MS Homo sapiens
67 RHOB 388
Proximity Label-MS Homo sapiens
68 HAX1  
Proximity Label-MS Homo sapiens
69 MRPS17 51373
Affinity Capture-MS Homo sapiens
70 ZC3H3  
Affinity Capture-MS Homo sapiens
71 PIK3R1 5295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 FLOT1 10211
Proximity Label-MS Homo sapiens
73 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
74 FGF11  
Affinity Capture-MS Homo sapiens
75 SIRT6  
Affinity Capture-MS Homo sapiens
76 GRB2 2885
Affinity Capture-MS Homo sapiens
77 NMB  
Affinity Capture-MS Homo sapiens
78 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 IRS2 8660
Affinity Capture-Western Homo sapiens
80 LCK 3932
Proximity Label-MS Homo sapiens
81 CREBBP  
Affinity Capture-MS Homo sapiens
82 ZNF764 92595
Affinity Capture-MS Homo sapiens
83 C11orf52 91894
Proximity Label-MS Homo sapiens
84 NUP50 10762
Affinity Capture-MS Homo sapiens
85 RAB2A 5862
Proximity Label-MS Homo sapiens
86 RAB7A 7879
Proximity Label-MS Homo sapiens
87 RAB11A 8766
Proximity Label-MS Homo sapiens
88 SHARPIN  
Affinity Capture-MS Homo sapiens
89 PIK3R2 5296
Affinity Capture-MS Homo sapiens
90 KRAS 3845
Proximity Label-MS Homo sapiens
91 POM121C  
Affinity Capture-MS Homo sapiens
92 PTGER4  
Two-hybrid Homo sapiens
93 IRS1 3667
Affinity Capture-Western Homo sapiens
94 CAV1 857
Proximity Label-MS Homo sapiens
95 PIK3R3 8503
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here