Gene description for Scrib
Gene name scribbled homolog (Drosophila)
Gene symbol Scrib
Other names/aliases AI118201
CRIB
Crc
SCRIB1
Scrb1
mKIAA0147
vartul
Species Mus musculus
 Database cross references - Scrib
ExoCarta ExoCarta_105782
Vesiclepedia VP_105782
Entrez Gene 105782
UniProt Q80U72  
 Scrib identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Scrib
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    morphogenesis of embryonic epithelium GO:0016331 IMP
    apoptotic process involved in morphogenesis GO:0060561 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    wound healing GO:0042060 IGI
    cochlear nucleus development GO:0021747 IGI
    cell migration GO:0016477 ISO
    asymmetric protein localization GO:0008105 IMP
    establishment of apical/basal cell polarity GO:0035089 ISO
    cell differentiation GO:0030154 IEA
    auditory receptor cell stereocilium organization GO:0060088 IGI
    cell proliferation GO:0008283 ISO
    synaptic vesicle endocytosis GO:0048488 IMP
    negative regulation of mitotic cell cycle GO:0045930 ISO
    neural tube closure GO:0001843 ISO
    single organismal cell-cell adhesion GO:0016337 ISO
    activation of GTPase activity GO:0090630 ISO
    synaptic vesicle targeting GO:0016080 IMP
    positive regulation of receptor recycling GO:0001921 ISO
    positive chemotaxis GO:0050918 ISO
    multicellular organismal development GO:0007275 IEA
    mammary gland duct morphogenesis GO:0060603 IMP
    protein localization to adherens junction GO:0071896 ISO
    auditory receptor cell morphogenesis GO:0002093 IGI
    astrocyte cell migration GO:0043615 ISO
Subcellular Localization
    cell leading edge GO:0031252 ISO
    Scrib-APC-beta-catenin complex GO:0034750 ISO
    cell projection GO:0042995 IEA
    presynaptic membrane GO:0042734 ISO
    cell GO:0005623 IMP
    basolateral plasma membrane GO:0016323 IDA
    nucleoplasm GO:0005654 ISO
    postsynaptic membrane GO:0045211 ISO
    membrane GO:0016020 IEA
    cell-cell junction GO:0005911 ISO
    extracellular exosome GO:0070062 ISO
    myelin sheath abaxonal region GO:0035748 IDA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 ISO
    cell-cell adherens junction GO:0005913 ISO
    cell junction GO:0030054 IEA
 Experiment description of studies that identified Scrib in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Scrib
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Scrib is involved
PathwayEvidenceSource
Asymmetric localization of PCP proteins IEA Reactome





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