Gene description for Acta2
Gene name actin, alpha 2, smooth muscle, aorta
Gene symbol Acta2
Other names/aliases 0610041G09Rik
Actvs
SMalphaA
a-SMA
alphaSMA
Species Mus musculus
 Database cross references - Acta2
ExoCarta ExoCarta_11475
Entrez Gene 11475
UniProt P62737  
 Acta2 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
 Gene ontology annotations for Acta2
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IPI
Biological Process
    vascular smooth muscle contraction GO:0014829 IMP
    regulation of blood pressure GO:0008217 IMP
    muscle contraction GO:0006936 ISO
Subcellular Localization
    actin cytoskeleton GO:0015629 ISO
    smooth muscle contractile fiber GO:0030485 IDA
    extracellular exosome GO:0070062 ISO
    cytoskeleton GO:0005856 IDA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Acta2 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Acta2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Acta2 is involved
PathwayEvidenceSource
Smooth Muscle Contraction IEA Reactome





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