Gene description for Pdia4
Gene name
protein disulfide isomerase family A, member 4
Gene symbol
Pdia4
Other names/aliases
ERp-72 Erp70 Erp72
Species
Rattus norvegicus
Database cross references - Pdia4
ExoCarta
ExoCarta_116598
Vesiclepedia
VP_116598
Entrez Gene
116598
UniProt
P38659
Pdia4 identified in exosomes derived from the following tissue/cell type
Hepatocytes
19367702
Reticulocytes
21828046
Gene ontology annotations for Pdia4
Experiment description of studies that identified Pdia4 in exosomes
1
Experiment ID
35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19367702
Organism
Rattus norvegicus
Experiment description
Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors
Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name
JPR
Publication year
2008
Sample
Hepatocytes
Sample name
Hepatocytes
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF] Western blotting
2
Experiment ID
97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D4 Rep 2
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.16-1.21 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
Protein-protein interactions for Pdia4
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
Hsp90b1
362862
Affinity Capture-Western
Rattus norvegicus
2
Hyou1
192235
Affinity Capture-Western
Rattus norvegicus
3
Hspa5
25617
Affinity Capture-Western
Rattus norvegicus
Affinity Capture-Western
Rattus norvegicus
4
Thbs1
445442
Affinity Capture-Western
Rattus norvegicus
5
Tg
Affinity Capture-Western
Rattus norvegicus
Affinity Capture-Western
Rattus norvegicus
View the network
image/svg+xml
Pathways in which Pdia4 is involved
No pathways found