Gene description for Pdia4
Gene name protein disulfide isomerase family A, member 4
Gene symbol Pdia4
Other names/aliases ERp-72
Erp70
Erp72
Species Rattus norvegicus
 Database cross references - Pdia4
ExoCarta ExoCarta_116598
Vesiclepedia VP_116598
Entrez Gene 116598
UniProt P38659  
 Pdia4 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Pdia4
Molecular Function
    protein disulfide isomerase activity GO:0003756 IBA
    protein disulfide isomerase activity GO:0003756 IDA
    protein disulfide isomerase activity GO:0003756 IEA
    protein disulfide isomerase activity GO:0003756 ISO
    integrin binding GO:0005178 ISO
    protein binding GO:0005515 IPI
    protein-disulfide reductase activity GO:0015035 IEA
    protein-disulfide reductase activity GO:0015035 ISO
Biological Process
    protein folding GO:0006457 IBA
    response to endoplasmic reticulum stress GO:0034976 IBA
    chaperone-mediated protein folding GO:0061077 IDA
    platelet aggregation GO:0070527 ISO
    blood coagulation, fibrin clot formation GO:0072378 ISO
    positive regulation of protein folding GO:1903334 IDA
Subcellular Localization
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum lumen GO:0005788 IEA
    smooth endoplasmic reticulum GO:0005790 IDA
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    endoplasmic reticulum chaperone complex GO:0034663 ISO
    melanosome GO:0042470 IEA
 Experiment description of studies that identified Pdia4 in exosomes
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pdia4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hsp90b1 362862
Affinity Capture-Western Rattus norvegicus
2 Hyou1 192235
Affinity Capture-Western Rattus norvegicus
3 Hspa5 25617
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
4 Thbs1 445442
Affinity Capture-Western Rattus norvegicus
5 Tg  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Pdia4 is involved
No pathways found





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