Gene description for Thbs1
Gene name thrombospondin 1
Gene symbol Thbs1
Other names/aliases TSP-1
Tsp1
Species Rattus norvegicus
 Database cross references - Thbs1
ExoCarta ExoCarta_445442
Entrez Gene 445442
 Thbs1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Thbs1
Molecular Function
    heparin binding GO:0008201 ISO
    fibronectin binding GO:0001968 ISO
    fibroblast growth factor binding GO:0017134 ISO
    laminin binding GO:0043236 ISO
    collagen V binding GO:0070052 ISO
    phosphatidylserine binding GO:0001786 ISO
    transforming growth factor beta binding GO:0050431 IDA
    calcium ion binding GO:0005509 IEA
    fibrinogen binding GO:0070051 ISO
    extracellular matrix binding GO:0050840 ISO
    integrin binding GO:0005178 ISO
    low-density lipoprotein particle binding GO:0030169 ISO
Biological Process
    positive regulation of macrophage activation GO:0043032 ISO
    response to testosterone GO:0033574 IEP
    cellular response to tumor necrosis factor GO:0071356 IEP
    response to endoplasmic reticulum stress GO:0034976 ISO
    growth plate cartilage development GO:0003417 ISO
    negative regulation of nitric oxide mediated signal transduction GO:0010751 ISO
    negative regulation of fibrinolysis GO:0051918 ISO
    negative regulation of cGMP-mediated signaling GO:0010754 ISO
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISO
    cell adhesion GO:0007155 IEA
    blood vessel morphogenesis GO:0048514 ISO
    positive regulation of blood coagulation GO:0030194 ISO
    inflammatory response GO:0006954 ISO
    positive regulation of chemotaxis GO:0050921 ISO
    cell cycle arrest GO:0007050 ISO
    cellular response to nitric oxide GO:0071732 ISO
    positive regulation of fibroblast migration GO:0010763 ISO
    negative regulation of blood vessel endothelial cell migration GO:0043537 ISO
    positive regulation of phosphorylation GO:0042327 ISO
    sprouting angiogenesis GO:0002040 ISO
    positive regulation of transforming growth factor beta production GO:0071636 IMP
    negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II GO:0002581 ISO
    negative regulation of endothelial cell migration GO:0010596 ISO
    positive regulation of endothelial cell apoptotic process GO:2000353 ISO
    negative regulation of endothelial cell chemotaxis GO:2001027 ISO
    positive regulation of cell-substrate adhesion GO:0010811 ISO
    positive regulation of protein kinase B signaling GO:0051897 ISO
    negative regulation of endothelial cell proliferation GO:0001937 ISO
    response to glucose GO:0009749 ISO
    immune response GO:0006955 ISO
    positive regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902043 ISO
    cellular response to growth factor stimulus GO:0071363 IEP
    behavioral response to pain GO:0048266 ISO
    response to mechanical stimulus GO:0009612 IMP
    response to magnesium ion GO:0032026 ISO
    positive regulation of endothelial cell migration GO:0010595 ISO
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of macrophage chemotaxis GO:0010759 ISO
    negative regulation of dendritic cell antigen processing and presentation GO:0002605 ISO
    positive regulation of translation GO:0045727 ISO
    chronic inflammatory response GO:0002544 ISO
    response to drug GO:0042493 ISO
    activation of MAPK activity GO:0000187 ISO
    response to calcium ion GO:0051592 ISO
    positive regulation of angiogenesis GO:0045766 ISO
    endocardial cushion development GO:0003197 ISO
    peptide cross-linking GO:0018149 ISO
    outflow tract morphogenesis GO:0003151 ISO
    positive regulation of blood vessel endothelial cell migration GO:0043536 ISO
    negative regulation of angiogenesis GO:0016525 ISO
    negative regulation of interleukin-12 production GO:0032695 ISO
    negative regulation of plasma membrane long-chain fatty acid transport GO:0010748 ISO
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 ISO
    engulfment of apoptotic cell GO:0043652 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of tumor necrosis factor biosynthetic process GO:0042535 ISO
    negative regulation of cell-matrix adhesion GO:0001953 ISO
    positive regulation of reactive oxygen species metabolic process GO:2000379 ISO
    regulation of cGMP metabolic process GO:0030823 ISO
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 ISO
    negative regulation of plasminogen activation GO:0010757 ISO
    cell migration GO:0016477 ISO
Subcellular Localization
    sarcoplasmic reticulum GO:0016529 ISO
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 ISO
    platelet alpha granule GO:0031091 ISO
    cell surface GO:0009986 ISO
    extracellular exosome GO:0070062 ISO
    extracellular matrix GO:0031012 ISO
    secretory granule GO:0030141 ISO
    fibrinogen complex GO:0005577 ISO
    external side of plasma membrane GO:0009897 ISO
    extracellular space GO:0005615 ISO
 Experiment description of studies that identified Thbs1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 99
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 100
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
10
Experiment ID 101
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Thbs1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Thbs1 is involved
No pathways found





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