Gene description for Apoe
Gene name apolipoprotein E
Gene symbol Apoe
Other names/aliases AI255918
Apo-E
Species Mus musculus
 Database cross references - Apoe
ExoCarta ExoCarta_11816
Entrez Gene 11816
UniProt P08226  
 Apoe identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
 Gene ontology annotations for Apoe
Molecular Function
    cholesterol transporter activity GO:0017127 IDA
    beta-amyloid binding GO:0001540 ISO
    protein homodimerization activity GO:0042803 ISO
    cholesterol binding GO:0015485 IBA
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    identical protein binding GO:0042802 ISO
    antioxidant activity GO:0016209 ISO
    metal chelating activity GO:0046911 ISO
    very-low-density lipoprotein particle receptor binding GO:0070326 ISO
    heparin binding GO:0008201 ISO
    protein binding GO:0005515 IPI
    phosphatidylcholine-sterol O-acyltransferase activator activity GO:0060228 ISO
    lipid binding GO:0008289 ISO
    receptor binding GO:0005102 ISO
    lipoprotein particle binding GO:0071813 IDA
    hydroxyapatite binding GO:0046848 ISO
    tau protein binding GO:0048156 ISO
    lipid transporter activity GO:0005319 ISO
    phospholipid binding GO:0005543 ISO
Biological Process
    positive regulation of neuron death GO:1901216 ISO
    lipid metabolic process GO:0006629 IGI
    cGMP-mediated signaling GO:0019934 ISO
    maintenance of location in cell GO:0051651 IMP
    regulation of Cdc42 protein signal transduction GO:0032489 ISO
    cholesterol homeostasis GO:0042632 ISO
    positive regulation of cholesterol efflux GO:0010875 ISO
    cholesterol catabolic process GO:0006707 IMP
    cardiovascular system development GO:0072358 IMP
    regulation of tau-protein kinase activity GO:1902947 ISO
    negative regulation of dendritic spine development GO:0061000 ISO
    negative regulation of postsynaptic membrane organization GO:1901627 ISO
    regulation of cholesterol transport GO:0032374 IBA
    long-chain fatty acid transport GO:0015909 ISO
    cholesterol biosynthetic process GO:0006695 IBA
    lipoprotein catabolic process GO:0042159 IMP
    negative regulation of endothelial cell proliferation GO:0001937 ISO
    negative regulation of blood vessel endothelial cell migration GO:0043537 ISO
    alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering GO:0097113 ISO
    regulation of axon extension GO:0030516 IBA
    nitric oxide mediated signal transduction GO:0007263 ISO
    very-low-density lipoprotein particle clearance GO:0034447 ISO
    positive regulation of postsynaptic membrane organization GO:1901628 ISO
    phospholipid efflux GO:0033700 ISO
    vasodilation GO:0042311 IMP
    chylomicron remnant clearance GO:0034382 ISO
    virion assembly GO:0019068 ISO
    G-protein coupled receptor signaling pathway GO:0007186 ISO
    protein localization GO:0008104 IMP
    lipoprotein metabolic process GO:0042157 ISO
    axon regeneration GO:0031103 IGI
    positive regulation of presynaptic membrane organization GO:1901631 ISO
    lipid transport GO:0006869 ISO
    cholesterol metabolic process GO:0008203 ISO
    negative regulation of cholesterol biosynthetic process GO:0045541 ISO
    high-density lipoprotein particle assembly GO:0034380 ISO
    positive regulation of membrane protein ectodomain proteolysis GO:0051044 ISO
    receptor-mediated endocytosis GO:0006898 ISO
    positive regulation of lipid transport across blood brain barrier GO:1903002 ISO
    negative regulation of platelet activation GO:0010544 ISO
    lipoprotein biosynthetic process GO:0042158 IGI
    negative regulation of lipid biosynthetic process GO:0051055 ISO
    cellular calcium ion homeostasis GO:0006874 IDA
    positive regulation of dendritic spine development GO:0060999 ISO
    low-density lipoprotein particle remodeling GO:0034374 IMP
    triglyceride catabolic process GO:0019433 IBA
    triglyceride metabolic process GO:0006641 ISO
    positive regulation of phospholipid efflux GO:1902995 ISO
    negative regulation of cholesterol efflux GO:0090370 ISO
    negative regulation of MAP kinase activity GO:0043407 ISO
    positive regulation of dendritic spine maintenance GO:1902952 ISO
    lipid homeostasis GO:0055088 IMP
    positive regulation of nitric-oxide synthase activity GO:0051000 ISO
    positive regulation of cGMP biosynthetic process GO:0030828 ISO
    response to oxidative stress GO:0006979 IMP
    N-methyl-D-aspartate receptor clustering GO:0097114 ISO
    artery morphogenesis GO:0048844 IGI
    response to dietary excess GO:0002021 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    negative regulation of lipid transport across blood brain barrier GO:1903001 ISO
    negative regulation of dendritic spine maintenance GO:1902951 ISO
    very-low-density lipoprotein particle remodeling GO:0034372 ISO
    positive regulation of neurofibrillary tangle assembly GO:1902998 ISO
    aging GO:0007568 ISO
    protein import GO:0017038 ISO
    negative regulation of blood coagulation GO:0030195 ISO
    negative regulation of beta-amyloid formation GO:1902430 ISO
    negative regulation of inflammatory response GO:0050728 IGI
    positive regulation of low-density lipoprotein particle receptor catabolic process GO:0032805 ISO
    transport GO:0006810 IEA
    regulation of beta-amyloid clearance GO:1900221 ISO
    negative regulation of neuron death GO:1901215 ISO
    high-density lipoprotein particle remodeling GO:0034375 ISO
    negative regulation of presynaptic membrane organization GO:1901630 ISO
    positive regulation of cholesterol esterification GO:0010873 ISO
    reverse cholesterol transport GO:0043691 ISO
    regulation of gene expression GO:0010468 IMP
    high-density lipoprotein particle clearance GO:0034384 ISO
    positive regulation of axon extension GO:0045773 ISO
    regulation of neuron death GO:1901214 ISO
    fatty acid homeostasis GO:0055089 ISO
    positive regulation of lipid biosynthetic process GO:0046889 ISO
    negative regulation of phospholipid efflux GO:1902999 ISO
    positive regulation by host of viral process GO:0044794 ISO
    cholesterol efflux GO:0033344 ISO
    positive regulation of catalytic activity GO:0043085 IMP
    positive regulation of beta-amyloid formation GO:1902004 ISO
Subcellular Localization
    high-density lipoprotein particle GO:0034364 ISO
    extracellular space GO:0005615 ISO
    blood microparticle GO:0072562 ISO
    Golgi apparatus GO:0005794 ISO
    cell surface GO:0009986 ISO
    nucleus GO:0005634 ISO
    very-low-density lipoprotein particle GO:0034361 ISO
    endoplasmic reticulum GO:0005783 ISO
    plasma membrane GO:0005886 ISO
    early endosome GO:0005769 ISO
    lysosome GO:0005764 ISO
    extracellular matrix GO:0031012 ISO
    extrinsic component of external side of plasma membrane GO:0031232 ISO
    endosome GO:0005768 ISO
    late endosome GO:0005770 ISO
    discoidal high-density lipoprotein particle GO:0034365 ISO
    neuronal cell body GO:0043025 ISO
    chylomicron GO:0042627 ISO
    intermediate-density lipoprotein particle GO:0034363 ISO
    membrane GO:0016020 ISO
    extracellular vesicle GO:1903561 ISO
    nuclear envelope GO:0005635 ISO
    extracellular exosome GO:0070062 ISO
    dendrite GO:0030425 ISO
    low-density lipoprotein particle GO:0034362 ISO
    extracellular region GO:0005576 IEA
    cell GO:0005623 IDA
    cytoplasm GO:0005737 ISO
    microtubule GO:0005874 ISO
 Experiment description of studies that identified Apoe in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Apoe
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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