Gene description for H3f3a
Gene name H3 histone, family 3A
Gene symbol H3f3a
Other names/aliases H3.3A
Species Mus musculus
 Database cross references - H3f3a
ExoCarta ExoCarta_15078
Vesiclepedia VP_15078
Entrez Gene 15078
UniProt P84244  
 H3f3a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 11390481    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for H3f3a
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 ISO
    nucleosomal DNA binding GO:0031492 ISO
Biological Process
    oocyte maturation GO:0001556 IDA
    osteoblast differentiation GO:0001649 IGI
    spermatogenesis GO:0007283 IMP
    single fertilization GO:0007338 IMP
    cell population proliferation GO:0008283 IGI
    pericentric heterochromatin formation GO:0031508 IGI
    subtelomeric heterochromatin formation GO:0031509 IGI
    multicellular organism growth GO:0035264 IMP
    muscle cell differentiation GO:0042692 IGI
    oogenesis GO:0048477 IGI
    negative regulation of chromosome condensation GO:1902340 IGI
Subcellular Localization
    chromosome, centromeric region GO:0000775 IDA
    chromosome, telomeric region GO:0000781 ISO
    chromatin GO:0000785 IDA
    nucleosome GO:0000786 ISO
    inner kinetochore GO:0000939 IDA
    Barr body GO:0001740 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    chromosome GO:0005694 IDA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified H3f3a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for H3f3a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Creb3l4  
Affinity Capture-Western Mus musculus
2 Cbx2  
Reconstituted Complex Mus musculus
Protein-peptide Mus musculus
3 Kmt2b  
Biochemical Activity Mus musculus
4 Kmt2c  
Biochemical Activity Mus musculus
5 Setd1b 208043
Biochemical Activity Mus musculus
6 Chd5  
Protein-peptide Mus musculus
7 Cbx7  
Far Western Mus musculus
Protein-peptide Mus musculus
8 Tfe3  
Affinity Capture-MS Mus musculus
9 Nfkb2  
Affinity Capture-Western Mus musculus
10 Hdac5  
Affinity Capture-Western Mus musculus
11 Ezh2  
Protein-peptide Mus musculus
12 Ehmt2  
Biochemical Activity Mus musculus
13 Aurka  
Affinity Capture-Western Mus musculus
14 Aurkb  
Biochemical Activity Mus musculus
15 Cbx5  
Reconstituted Complex Mus musculus
16 Kdm6b  
Biochemical Activity Mus musculus
17 Cbx8  
Protein-peptide Mus musculus
18 Bptf 207165
Affinity Capture-Western Mus musculus
19 Cbx3 12417
Protein-peptide Mus musculus
20 Kmt2d  
Biochemical Activity Mus musculus
21 Hdac3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
22 Chd1  
Protein-peptide Mus musculus
23 Uhrf1  
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
Protein-peptide Mus musculus
24 Cbx6  
Protein-peptide Mus musculus
25 Cbx4  
Protein-peptide Mus musculus
26 Setd1a  
Biochemical Activity Mus musculus
27 Ing2  
Co-crystal Structure Mus musculus
Reconstituted Complex Mus musculus
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