Gene description for Nucb1
Gene name nucleobindin 1
Gene symbol Nucb1
Other names/aliases B230337F23Rik
C77483
Calnuc
MTEST82
Nucb
Species Mus musculus
 Database cross references - Nucb1
ExoCarta ExoCarta_18220
Vesiclepedia VP_18220
Entrez Gene 18220
UniProt Q02819  
 Nucb1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nucb1
Molecular Function
    G-protein alpha-subunit binding GO:0001965 ISO
    G-protein alpha-subunit binding GO:0001965 ISS
    DNA binding GO:0003677 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 ISO
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
Biological Process
    small GTPase-mediated signal transduction GO:0007264 ISO
    small GTPase-mediated signal transduction GO:0007264 ISS
    regulation of protein targeting GO:1903533 ISO
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular region GO:0005576 ISO
    extracellular space GO:0005615 ISO
    early endosome GO:0005769 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IBA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    Golgi apparatus GO:0005794 IEA
    Golgi-associated vesicle GO:0005798 ISO
    trans-Golgi network GO:0005802 ISO
    membrane GO:0016020 IEA
    Golgi cisterna membrane GO:0032580 ISO
    extrinsic component of Golgi membrane GO:0090498 ISO
    lumenal side of Golgi membrane GO:0098547 ISO
 Experiment description of studies that identified Nucb1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nucb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Wars 22375
Co-fractionation Mus musculus
3 Ptgs2 19225
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
4 Ndn  
Two-hybrid Mus musculus
5 Ptgs1 19224
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
6 Iqcb1  
Affinity Capture-MS Mus musculus
7 Kcnma1  
Affinity Capture-MS Mus musculus
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