Gene description for Ptgir
Gene name prostaglandin I receptor (IP)
Gene symbol Ptgir
Other names/aliases IP
PGI2
Species Mus musculus
 Database cross references - Ptgir
ExoCarta ExoCarta_19222
Vesiclepedia VP_19222
Entrez Gene 19222
UniProt P43252  
 Ptgir identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Ptgir
Molecular Function
    G protein-coupled receptor activity GO:0004930 IEA
    prostacyclin receptor activity GO:0016501 IBA
Biological Process
    inflammatory response GO:0006954 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISO
    positive regulation of cytosolic calcium ion concentration GO:0007204 IBA
    negative regulation of platelet-derived growth factor receptor signaling pathway GO:0010642 IMP
    response to lipopolysaccharide GO:0032496 IMP
    negative regulation of smooth muscle cell proliferation GO:0048662 IBA
    negative regulation of smooth muscle cell proliferation GO:0048662 IMP
    negative regulation of smooth muscle cell proliferation GO:0048662 ISO
Subcellular Localization
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Ptgir in exosomes
1
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ptgir
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gna11 14672
Affinity Capture-Western Mus musculus
2 Gnai1  
Affinity Capture-Western Mus musculus
3 Gnas 14683
Affinity Capture-Western Mus musculus
4 PDZK1 5174
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
View the network image/svg+xml



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