Gene description for Shmt1
Gene name serine hydroxymethyltransferase 1 (soluble)
Gene symbol Shmt1
Other names/aliases AI324848
AI385541
C81125
Shmt
mshmt
mshmt1
mshmt2
Species Mus musculus
 Database cross references - Shmt1
ExoCarta ExoCarta_20425
Entrez Gene 20425
UniProt P50431  
 Shmt1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Shmt1
Molecular Function
    transferase activity GO:0016740 IEA
    identical protein binding GO:0042802 ISO
    catalytic activity GO:0003824 IEA
    pyridoxal phosphate binding GO:0030170 ISO
    protein homodimerization activity GO:0042803 ISO
    glycine hydroxymethyltransferase activity GO:0004372 ISO
    L-allo-threonine aldolase activity GO:0008732 ISO
    amino acid binding GO:0016597 ISO
Biological Process
    protein tetramerization GO:0051262 ISO
    one-carbon metabolic process GO:0006730 IEA
    protein homotetramerization GO:0051289 ISO
    folic acid metabolic process GO:0046655 ISO
    glycine biosynthetic process from serine GO:0019264 ISO
    purine nucleobase biosynthetic process GO:0009113 ISO
    glycine biosynthetic process GO:0006545 ISO
    L-serine catabolic process GO:0006565 ISO
    glycine metabolic process GO:0006544 ISO
    L-serine metabolic process GO:0006563 IEA
Subcellular Localization
    cytosol GO:0005829 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IDA
    nucleoplasm GO:0005654 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Shmt1 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Shmt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Shmt1 is involved
PathwayEvidenceSource
Carnitine synthesis IEA Reactome
Metabolism of folate and pterines IEA Reactome





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