Gene description for NEGR1
Gene name neuronal growth regulator 1
Gene symbol NEGR1
Other names/aliases DMML2433
IGLON4
KILON
Ntra
Species Homo sapiens
 Database cross references - NEGR1
ExoCarta ExoCarta_257194
Vesiclepedia VP_257194
Entrez Gene 257194
HGNC 17302
MIM 613173
UniProt Q7Z3B1  
 NEGR1 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NEGR1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    brain development GO:0007420 IEA
    locomotory behavior GO:0007626 IEA
    feeding behavior GO:0007631 IEA
    positive regulation of neuron projection development GO:0010976 IEA
    social behavior GO:0035176 IEA
    cholesterol homeostasis GO:0042632 IEA
    fat cell differentiation GO:0045444 IEA
    positive regulation of saliva secretion GO:0046878 IEA
    neuron projection morphogenesis GO:0048812 IEA
    positive regulation of calcium-mediated signaling GO:0050850 IEA
    regulation of synapse assembly GO:0051963 IEA
    skeletal muscle organ development GO:0060538 IEA
    cell-cell adhesion GO:0098609 IEA
    lipid droplet formation GO:0140042 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    plasma membrane GO:0005886 TAS
    postsynaptic density GO:0014069 IEA
    dendrite GO:0030425 IEA
    neuronal cell body GO:0043025 IEA
    side of membrane GO:0098552 IEA
 Experiment description of studies that identified NEGR1 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NEGR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 E2F4  
Affinity Capture-MS Homo sapiens
2 Htt  
Affinity Capture-MS Mus musculus
3 KIF23 9493
Affinity Capture-MS Homo sapiens
4 KIF14 9928
Affinity Capture-MS Homo sapiens
5 GLMN 11146
Affinity Capture-MS Homo sapiens
6 ECT2 1894
Affinity Capture-MS Homo sapiens
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