Experiment description of studies that identified RNU5A-1 in exosomes

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule snRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule snRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

3 Experiment ID 222 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule snRNA Identification method RT-PCR PubMed ID 23663360     Organism Homo sapiens Experiment description Characterization of human plasma-derived exosomal RNAs by deep sequencing Authors Huang X, Yuan T, Tschannen M, Sun Z, Jacob H, Du M, Liang M, Dittmar RL, Liu Y, Liang M, Kohli M, Thibodeau SN, Boardman L, Wang L Journal name BMC Genomics Publication year 2013 Sample Plasma Sample name Plasma - Normal Isolation/purification methods Exoquick Flotation density - Molecules identified in the study miRNA tRNA snRNA snoRNA rRNA piRNA lncRNA Methods used in the study RT-PCR

Protein-protein interactions for RNU5A-1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBAP2L 9898 Affinity Capture-RNA Homo sapiens 2 EFTUD2 9343 Affinity Capture-RNA Homo sapiens 3 ZMYND11 10771 Affinity Capture-RNA Homo sapiens 4 C9orf78 51759 Affinity Capture-RNA Homo sapiens 5 G3BP1 10146 Affinity Capture-RNA Homo sapiens 6 ILF3 3609 Affinity Capture-RNA Homo sapiens View the network

Pathways in which RNU5A-1 is involved

No pathways found