Gene description for RNU5A-1
Gene name RNA, U5A small nuclear 1
Gene symbol RNU5A-1
Other names/aliases RNU5
RNU5A
RNU5C
U5A
U5B1
Species Homo sapiens
 Database cross references - RNU5A-1
ExoCarta ExoCarta_26831
Entrez Gene 26831
HGNC 10211
MIM 180691
 RNU5A-1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Plasma 23663360    
 Gene ontology annotations for RNU5A-1
 Experiment description of studies that identified RNU5A-1 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule snRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule snRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
3
Experiment ID 222
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule snRNA
Identification method RT-PCR
PubMed ID 23663360    
Organism Homo sapiens
Experiment description Characterization of human plasma-derived exosomal RNAs by deep sequencing
Authors Huang X, Yuan T, Tschannen M, Sun Z, Jacob H, Du M, Liang M, Dittmar RL, Liu Y, Liang M, Kohli M, Thibodeau SN, Boardman L, Wang L
Journal name BMC Genomics
Publication year 2013
Sample Plasma
Sample name Plasma - Normal
Isolation/purification methods Exoquick
Flotation density -
Molecules identified in the study miRNA
tRNA
snRNA
snoRNA
rRNA
piRNA
lncRNA
Methods used in the study RT-PCR
 Protein-protein interactions for RNU5A-1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RNU5A-1 is involved
No pathways found





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