Gene description for ANGPTL3
Gene name angiopoietin-like 3
Gene symbol ANGPTL3
Other names/aliases ANG-5
ANGPT5
ANL3
FHBL2
Species Homo sapiens
 Database cross references - ANGPTL3
ExoCarta ExoCarta_27329
Vesiclepedia VP_27329
Entrez Gene 27329
HGNC 491
MIM 604774
UniProt Q9Y5C1  
 ANGPTL3 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Prostate cancer cells 25844599    
 Gene ontology annotations for ANGPTL3
Molecular Function
    enzyme inhibitor activity GO:0004857 ISS
    phospholipase inhibitor activity GO:0004859 IBA
    phospholipase inhibitor activity GO:0004859 IDA
    integrin binding GO:0005178 IPI
    growth factor activity GO:0008083 IDA
    heparin binding GO:0008201 IEA
Biological Process
    angiogenesis GO:0001525 IEA
    glycerol metabolic process GO:0006071 IDA
    fatty acid metabolic process GO:0006631 IDA
    phospholipid metabolic process GO:0006644 IDA
    cell-matrix adhesion GO:0007160 IPI
    signal transduction GO:0007165 IDA
    integrin-mediated signaling pathway GO:0007229 NAS
    cholesterol metabolic process GO:0008203 IDA
    phospholipid catabolic process GO:0009395 IBA
    phospholipid catabolic process GO:0009395 IDA
    response to hormone GO:0009725 IEA
    negative regulation of phospholipase activity GO:0010519 IDA
    lipid storage GO:0019915 IDA
    positive regulation of cell migration GO:0030335 IDA
    cholesterol homeostasis GO:0042632 IBA
    cholesterol homeostasis GO:0042632 IDA
    positive regulation of angiogenesis GO:0045766 IDA
    artery morphogenesis GO:0048844 ISS
    positive regulation of lipid catabolic process GO:0050996 IDA
    negative regulation of lipoprotein lipase activity GO:0051005 IDA
    lipid homeostasis GO:0055088 IDA
    acylglycerol homeostasis GO:0055090 IDA
    phospholipid homeostasis GO:0055091 IBA
    phospholipid homeostasis GO:0055091 IDA
    triglyceride homeostasis GO:0070328 IBA
    triglyceride homeostasis GO:0070328 IGI
    triglyceride homeostasis GO:0070328 ISS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    early endosome GO:0005769 IEA
    Golgi apparatus GO:0005794 IEA
    cell surface GO:0009986 IDA
    lamellipodium GO:0030027 IEA
    collagen-containing extracellular matrix GO:0062023 IBA
 Experiment description of studies that identified ANGPTL3 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for ANGPTL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC91 55297
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 FBXO28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 UBC 7316
Reconstituted Complex Homo sapiens
4 GULP1 51454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 BIRC6 57448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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