Gene description for Flo2
Gene name Flotillin 2
Gene symbol Flo2
Other names/aliases CG11547
CG14409
CG32593
DmFlo2
DmelCG32593
FLO2
FLO[Dm]-2
Flo
Flo-2
flo2
reggie-1
Species Drosophila melanogaster
 Database cross references - Flo2
ExoCarta ExoCarta_32425
Vesiclepedia VP_32425
Entrez Gene 32425
UniProt O61492  
 Flo2 identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Flo2
Molecular Function
    structural molecule activity GO:0005198 NAS
Biological Process
    cell adhesion GO:0007155 NAS
    melanotic encapsulation of foreign target GO:0035011 IMP
    positive regulation of filopodium assembly GO:0051491 IDA
Subcellular Localization
    cytoplasm GO:0005737 HDA
    cytosol GO:0005829 HDA
    plasma membrane GO:0005886 HDA
    flotillin complex GO:0016600 NAS
 Experiment description of studies that identified Flo2 in sEVs
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Flo2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 dos  
Affinity Capture-MS Drosophila melanogaster
2 Vps24 40542
Affinity Capture-MS Drosophila melanogaster
3 fra  
Affinity Capture-MS Drosophila melanogaster
Affinity Capture-Western Drosophila melanogaster
4 mib2 35170
Affinity Capture-MS Drosophila melanogaster
5 Syb  
Affinity Capture-MS Drosophila melanogaster
6 CG6259 39964
Affinity Capture-MS Drosophila melanogaster
7 chico  
Affinity Capture-MS Drosophila melanogaster
8 Flo1  
Affinity Capture-MS Drosophila melanogaster
9 RpS27A  
Affinity Capture-Western Drosophila melanogaster
10 Tom20  
Proximity Label-MS Drosophila melanogaster
11 CHMP2B 38599
Affinity Capture-MS Drosophila melanogaster
12 shrb  
Affinity Capture-MS Drosophila melanogaster
13 HSPC300  
Affinity Capture-MS Drosophila melanogaster
14 CG7668  
Affinity Capture-MS Drosophila melanogaster
15 CG10103 38750
Affinity Capture-MS Drosophila melanogaster
16 zuc  
Proximity Label-MS Drosophila melanogaster
17 CG10732  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml



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