Gene description for CG6259
Gene name CG6259 gene product from transcript CG6259-RB
Gene symbol CG6259
Other names/aliases Chmp5
DmelCG6259
VPS60
anon-74EFb
bs36h07.y1
Species Drosophila melanogaster
 Database cross references - CG6259
ExoCarta ExoCarta_39964
Vesiclepedia VP_39964
Entrez Gene 39964
UniProt Q9VVI9  
 CG6259 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for CG6259
Biological Process
    symbiont entry into host cell GO:0046718 HMP
Subcellular Localization
    ESCRT III complex GO:0000815 NAS
 Experiment description of studies that identified CG6259 in exosomes
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CG6259
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 dos  
Affinity Capture-MS Drosophila melanogaster
2 Vps24 40542
Affinity Capture-MS Drosophila melanogaster
3 Vps2 43164
Affinity Capture-MS Drosophila melanogaster
4 mib2 35170
Affinity Capture-MS Drosophila melanogaster
5 CG10732  
Affinity Capture-MS Drosophila melanogaster
6 chico  
Affinity Capture-MS Drosophila melanogaster
7 Flo1  
Affinity Capture-MS Drosophila melanogaster
8 CG14985 38470
Two-hybrid Drosophila melanogaster
9 HSPC300  
Affinity Capture-MS Drosophila melanogaster
10 CG7668  
Affinity Capture-MS Drosophila melanogaster
11 CHMP2B 38599
Affinity Capture-MS Drosophila melanogaster
12 shrb  
Affinity Capture-MS Drosophila melanogaster
13 Chmp1  
Affinity Capture-MS Drosophila melanogaster
14 Vps4  
Affinity Capture-MS Drosophila melanogaster
15 CG10103 38750
Affinity Capture-MS Drosophila melanogaster
16 Flo2 32425
Affinity Capture-MS Drosophila melanogaster
17 CG3528  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml



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