Gene description for Hist1h1e
Gene name histone cluster 1, H1e
Gene symbol Hist1h1e
Other names/aliases H1-4
H1.4
H1e
H1f4
H1s-4
H1var2
Species Mus musculus
 Database cross references - Hist1h1e
ExoCarta ExoCarta_50709
Vesiclepedia VP_50709
Entrez Gene 50709
UniProt P43274  
 Hist1h1e identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hist1h1e
Molecular Function
    nucleotide binding GO:0000166 ISO
    DNA binding GO:0003677 IDA
    DNA binding GO:0003677 ISO
    double-stranded DNA binding GO:0003690 IBA
    double-stranded DNA binding GO:0003690 IEA
    double-stranded DNA binding GO:0003690 ISO
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    AMP binding GO:0016208 IEA
    AMP binding GO:0016208 ISO
    structural constituent of chromatin GO:0030527 IMP
    chromatin DNA binding GO:0031490 IEA
    chromatin DNA binding GO:0031490 ISO
    nucleosomal DNA binding GO:0031492 IBA
    dATP binding GO:0032564 IEA
    dATP binding GO:0032564 ISO
    histone deacetylase binding GO:0042826 IEA
    histone deacetylase binding GO:0042826 ISO
    ADP binding GO:0043531 IEA
    ADP binding GO:0043531 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    chromatin organization GO:0006325 IMP
    nucleosome assembly GO:0006334 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    chromosome condensation GO:0030261 IBA
    negative regulation of DNA recombination GO:0045910 IBA
Subcellular Localization
    nucleosome GO:0000786 IEA
    nucleosome GO:0000786 ISO
    euchromatin GO:0000791 IBA
    heterochromatin GO:0000792 IEA
    heterochromatin GO:0000792 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified Hist1h1e in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hist1h1e
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hist1h1e 50709
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Cbx5  
Protein-peptide Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
3 Cbx1  
Protein-peptide Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Lmna 16905
Affinity Capture-MS Mus musculus
7 Traf3  
Proximity Label-MS Mus musculus
8 Tfe3  
Affinity Capture-MS Mus musculus
9 Tia1  
Affinity Capture-MS Mus musculus
10 Ddb1 13194
Affinity Capture-MS Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 Cbx3 12417
Protein-peptide Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
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