ExoCarta: Gene summary

Credits | Contact

Gene description for Tceb1
Gene name	transcription elongation factor B (SIII), polypeptide 1
Gene symbol	Tceb1
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Tceb1
ExoCarta	ExoCarta_64525
Entrez Gene	64525
UniProt	P83941
Tceb1 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells	20124479
Pancreatic cancer cells	20124479

Gene ontology annotations for Tceb1

Molecular Function
	ubiquitin-protein transferase activity	GO:0004842	IC
	protein complex binding	GO:0032403	IPI
Biological Process
	protein ubiquitination involved in ubiquitin-dependent protein catabolic process	GO:0042787	IBA
	positive regulation of transcription elongation from RNA polymerase II promoter	GO:0032968	IDA
	transcription, DNA-templated	GO:0006351	IEA
Subcellular Localization
	elongin complex	GO:0070449	ISO
	VCB complex	GO:0030891	IDA

Experiment description of studies that identified Tceb1 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ HSP70\|GAPDH	EV Cytosolic markers
✔ CD9\|CD151	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

mrna

Identification method

RT-PCR
Microarray

PubMed ID

20124479

Organism

Rattus norvegicus

Experiment description

Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.

Authors

Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.

Journal name

Publication year

2010

Sample

Pancreatic cancer cells

Sample name

Isolation/purification methods

Differential centrifugation
Sucrose density gradient

Flotation density

1.14-1.17 g/mL

Molecules identified in the study

Protein
mRNA

Methods used in the study

RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]