Gene description for CHST3
Gene name carbohydrate (chondroitin 6) sulfotransferase 3
Gene symbol CHST3
Other names/aliases C6ST
C6ST1
HSD
Species Homo sapiens
 Database cross references - CHST3
ExoCarta ExoCarta_9469
Vesiclepedia VP_9469
Entrez Gene 9469
HGNC 1971
MIM 603799
UniProt Q7LGC8  
 CHST3 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CHST3
Molecular Function
    N-acetylglucosamine 6-O-sulfotransferase activity GO:0001517 IBA
    chondroitin 6-sulfotransferase activity GO:0008459 IBA
    chondroitin 6-sulfotransferase activity GO:0008459 IDA
    chondroitin 6-sulfotransferase activity GO:0008459 TAS
    proteoglycan sulfotransferase activity GO:0050698 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    N-acetylglucosamine metabolic process GO:0006044 IBA
    sulfur compound metabolic process GO:0006790 IDA
    chondroitin sulfate biosynthetic process GO:0030206 IBA
    chondroitin sulfate biosynthetic process GO:0030206 IDA
    chondroitin sulfate biosynthetic process GO:0030206 IMP
    chondroitin sulfate biosynthetic process GO:0030206 TAS
    T cell homeostasis GO:0043029 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
 Experiment description of studies that identified CHST3 in sEVs
1
Experiment ID 834
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CHST3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OFD1 8481
Affinity Capture-MS Homo sapiens
2 CLEC12B  
Affinity Capture-MS Homo sapiens
3 PDGFRA 5156
Affinity Capture-MS Homo sapiens
4 MSMB  
Affinity Capture-MS Homo sapiens
5 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 AVP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
8 C1QTNF7  
Affinity Capture-MS Homo sapiens
9 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 C1orf54  
Affinity Capture-MS Homo sapiens
11 CRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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