Gene description for ARHGEF12
Gene name Rho guanine nucleotide exchange factor (GEF) 12
Gene symbol ARHGEF12
Other names/aliases LARG
PRO2792
Species Homo sapiens
 Database cross references - ARHGEF12
ExoCarta ExoCarta_23365
Vesiclepedia VP_23365
Entrez Gene 23365
HGNC 14193
MIM 604763
UniProt Q9NZN5  
 ARHGEF12 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for ARHGEF12
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    G protein-coupled receptor binding GO:0001664 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
Biological Process
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    Rho protein signal transduction GO:0007266 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ARHGEF12 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ARHGEF12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 PTPRF 5792
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
3 PRDX4 10549
Affinity Capture-MS Homo sapiens
4 ANLN 54443
Affinity Capture-MS Homo sapiens
5 KLHL20  
Biochemical Activity Homo sapiens
6 TXLNA 200081
Affinity Capture-MS Homo sapiens
7 SMC3 9126
Affinity Capture-MS Homo sapiens
8 ARRB2 409
Affinity Capture-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 GNA12 2768
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
11 GIPC1 10755
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SMARCB1 6598
Affinity Capture-MS Homo sapiens
13 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CSNK1A1L 122011
Affinity Capture-MS Homo sapiens
15 PSMD6 9861
Affinity Capture-MS Homo sapiens
16 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SATB2  
Proximity Label-MS Homo sapiens
18 ARHGEF11 9826
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 LDHB 3945
Affinity Capture-MS Homo sapiens
20 RHOC 389
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SH3BP4 23677
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 DEPDC1B 55789
Affinity Capture-MS Homo sapiens
23 PSMD13 5719
Affinity Capture-MS Homo sapiens
24 PLEKHG4B 153478
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CALM2 805
Affinity Capture-MS Homo sapiens
26 KRT8 3856
Proximity Label-MS Homo sapiens
27 Itgb1 16412
Affinity Capture-MS Mus musculus
28 EZR 7430
Proximity Label-MS Homo sapiens
29 PSMC2 5701
Affinity Capture-MS Homo sapiens
30 SSX3  
Affinity Capture-MS Homo sapiens
31 CCDC183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CALM1 801
Affinity Capture-MS Homo sapiens
33 CUL3 8452
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
34 C7orf76  
Affinity Capture-MS Homo sapiens
35 DCTN2 10540
Proximity Label-MS Homo sapiens
36 PLXNB1 5364
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
37 IGF1R 3480
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 METTL21B  
Affinity Capture-MS Homo sapiens
39 Arhgap15  
Affinity Capture-MS Mus musculus
40 GAN 8139
Affinity Capture-MS Homo sapiens
41 OCLN 100506658
Proximity Label-MS Homo sapiens
42 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 SSBP1 6742
Affinity Capture-MS Homo sapiens
44 PLXNB2 23654
Affinity Capture-Western Homo sapiens
45 Espl1  
Affinity Capture-MS Mus musculus
46 TEC 7006
Phenotypic Enhancement Homo sapiens
Biochemical Activity Homo sapiens
47 PFN1 5216
Proximity Label-MS Homo sapiens
48 TRMT61A 115708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RPS6KA3 6197
Affinity Capture-MS Homo sapiens
50 PTP4A3  
Affinity Capture-MS Homo sapiens
51 ANKFY1 51479
Proximity Label-MS Homo sapiens
52 CDH1 999
Proximity Label-MS Homo sapiens
53 MYO6 4646
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
54 RCL1 10171
Affinity Capture-MS Homo sapiens
55 NTRK1 4914
Affinity Capture-MS Homo sapiens
56 PXN 5829
Proximity Label-MS Homo sapiens
57 FLOT1 10211
Proximity Label-MS Homo sapiens
58 DYDC2  
Affinity Capture-MS Homo sapiens
59 IMPDH2 3615
Proximity Label-MS Homo sapiens
60 GNA13 10672
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
61 CALM3 808
Affinity Capture-MS Homo sapiens
62 RHOB 388
Proximity Label-MS Homo sapiens
63 ARHGEF12 23365
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
64 RHOA 387
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
65 ERLEC1 27248
Affinity Capture-MS Homo sapiens
66 MALSU1  
Affinity Capture-MS Homo sapiens
67 ARHGEF40 55701
Proximity Label-MS Homo sapiens
68 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
69 CYLD  
Affinity Capture-Western Homo sapiens
70 GIPC2 54810
Proximity Label-MS Homo sapiens
71 UBXN6 80700
Affinity Capture-MS Homo sapiens
72 PSMD3 5709
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here